The Candida albicans gene for mRNA 5-cap methyltransferase: identification of additional residues essential for catalysis.

@article{YamadaOkabe1999TheCA,
  title={The Candida albicans gene for mRNA 5-cap methyltransferase: identification of additional residues essential for catalysis.},
  author={T. Yamada-Okabe and T. Mio and Y. Kashima and M. Matsui and M. Arisawa and H. Yamada-Okabe},
  journal={Microbiology},
  year={1999},
  volume={145 ( Pt 11)},
  pages={
          3023-33
        }
}
The 5'-cap structure of eukaryotic mRNA is methylated at the terminal guanosine by RNA (guanine-N7-)-methyltransferase (cap MTase). Saccharomyces cerevisiae ABD1 (ScABD1) and human hMet (also called CMT1) genes are responsible for this enzyme. The ABD1 homologue was cloned from the pathogenic fungus Candida albicans and named C. albicans ABD1 (CaABD1). When expressed as a fusion with glutathione S-transferase (GST), CaAbd1p displayed cap MTase activity in vitro and rescued S. cerevisiae… Expand
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Results indicate that the failure of the human TPase activity to complement an S. cerevisiae cet1Δ null mutation is attributable, at least in part, to the inability of thehuman capping enzyme to associate with the yeast GTase, and that the physical association of GTase and TPase is essential for the function of the capping enzymes in vivo. Expand
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TLDR
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