The Caco-2 cell monolayers as an intestinal metabolism model: metabolism of dipeptide Phe-Pro.

@article{Hu1994TheCC,
  title={The Caco-2 cell monolayers as an intestinal metabolism model: metabolism of dipeptide Phe-Pro.},
  author={Ming Hu and Jiyue Chen and David L. Tran and Yani Zhu and Giacomo Leonardo},
  journal={Journal of drug targeting},
  year={1994},
  volume={2 1},
  pages={79-89}
}
The metabolism of Phe-Pro was investigated in Caco-2 cell monolayers, a model of small intestinal epithelium. The results indicate that the majority of Phe-Pro was hydrolyzed during passage from the apical (AP) to basolateral (BL) side. The enzyme responsible for the hydrolysis is prolidase, a cytosolic enzyme. Through kinetic studies of a supernatant enzyme preparation, a Km of 30.4 microM and Vmax of 38.9 nmol/min per mg of protein were obtained. The enzyme catalyzed hydrolysis was inhibited… CONTINUE READING
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