The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics

  title={The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics},
  author={Y. Sambuy and I. Angelis and G. Ranaldi and M. Scarino and A. Stammati and F. Zucco},
  journal={Cell Biology and Toxicology},
The human intestinal Caco-2 cell line has been extensively used over the last twenty years as a model of the intestinal barrier. The parental cell line, originally obtained from a human colon adenocarcinoma, undergoes in culture a process of spontaneous differentiation that leads to the formation of a monolayer of cells, expressing several morphological and functional characteristics of the mature enterocyte. Culture-related conditions were shown to influence the expression of these… Expand
Good Caco-2 cell culture practices.
The Caco-2 cell culture protocol here described has been recently optimized in the laboratory, producing a homogeneous and highly polarized monolayer of cells which display many of the characteristics of the intestinal enterocytes. Expand
A protocol for differentiation of human intestinal Caco-2 cells in asymmetric serum-containing medium.
It is shown that addition of serum only to the BL medium appears to be sufficient to allow differentiation of Caco-2 cells, as monitored by morphology, monolayer permeability and alkaline phosphatase activity, compared to standard conditions using 10% FBS supplement in both AP and BL media (asymmetric protocol). Expand
Cell growing density affects the structural and functional properties of Caco‐2 differentiated monolayer
It is suggested that cell growing density could influence a number of morphological and physiological properties of differentiated Caco‐2 cells and these effects must be taken in account when these cells are used as intestinal model. Expand
An Inter-laboratory Study to Evaluate the Effects of Medium Composition on the Differentiation and Barrier Function of Caco-2 Cell Lines
Caco-2/TC7 cells performed better than the other three cell lines, both in terms of reproducibility and performance, exhibiting low TEER and mannitol passage, and high alkaline phosphatase activity. Expand
GM1 expression in caco-2 cells: characterisation of a fundamental passage-dependent transformation of a cell line.
The novel GM1 passage-dependent cell transformation described here shows that careful monitoring is required when performing in vitro cell studies, and to guarantee consistent and valid experimental data, GM1 expression and the loss of differentiation characteristics should be carefully monitored and the use of fresh cultures should be standard practice. Expand
T84 monolayers are superior to Caco-2 as a model system of colonocytes
Colonic adenocarcinoma-derived Caco-2 and T84 epithelial cell lines are frequently used as in vitro model systems of functional epithelial barriers. Both are utilised interchangeably despite evidenceExpand
A tunable Caco-2/HT29-MTX co-culture model mimicking variable permeabilities of the human intestine obtained by an original seeding procedure.
  • A. Béduneau, Camille Tempesta, +4 authors A. Lamprecht
  • Chemistry, Medicine
  • European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
  • 2014
The HT29-MTX seeding day was a key factor to set up an in vitro intestinal model with tailor-made barrier properties in terms of P-gp expression and paracellular permeability. Expand
Differentiation of Caco-2 cells requires both transcriptional and post-translational down-regulation of Myc.
A strong correlation between Myc protein downregulation and the expression of the transcription factor Cdx2 is found, suggesting the existence of a regulative link between these two proteins. Expand
Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis
The recently developed method for the culture of intestinal epithelial spheroids is adapted to establish primary epithelial cell monolayers from the colon of multiple genetic mouse strains, allowing the use of primary cells for studies of intestinal physiology. Expand
Morphofunctional properties of a differentiated Caco2/HT-29 co-culture as an in vitro model of human intestinal epithelium
This Caco2/HT-29 co-culture could be considered a versatile and suitable in vitro model of human intestinal epithelium for the presence of more than one prevalent intestinal cell type, by means of a minimum of 6 to a maximum of 14 post-confluence days obtained without the need of particular inducers of subclones and growth support to reach an intestinal differentiated phenotype. Expand