The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

@article{Garneau2010TheCB,
  title={The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA},
  author={Josiane E. Garneau and Marie-{\`E}ve Dupuis and M. Villion and D. A. Romero and R. Barrangou and P. Boyaval and C. Fremaux and P. Horvath and A. H. Magad{\'a}n and S. Moineau},
  journal={Nature},
  year={2010},
  volume={468},
  pages={67-71}
}
  • Josiane E. Garneau, Marie-Ève Dupuis, +7 authors S. Moineau
  • Published 2010
  • Medicine, Biology
  • Nature
  • Bacteria and Archaea have developed several defence strategies against foreign nucleic acids such as viral genomes and plasmids. [...] Key Result Here we show that the Streptococcus thermophilus CRISPR1/Cas system can also naturally acquire spacers from a self-replicating plasmid containing an antibiotic-resistance gene, leading to plasmid loss. Acquired spacers that match antibiotic-resistance genes provide a novel means to naturally select bacteria that cannot uptake and disseminate such genes.Expand Abstract

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