The C terminus of component C2II of Clostridium botulinum C2 toxin is essential for receptor binding.

@article{Blcker2000TheCT,
  title={The C terminus of component C2II of Clostridium botulinum C2 toxin is essential for receptor binding.},
  author={Dagmar Bl{\"o}cker and Holger Barth and Elke Maier and Roland Benz and Joseph T. Barbieri and Klaus Aktories},
  journal={Infection and immunity},
  year={2000},
  volume={68 8},
  pages={4566-73}
}
The binary Clostridium botulinum C2 toxin consists of two separate proteins, the binding component C2II (80.5 kDa) and the actin-ADP-ribosylating enzyme component C2I (49.4 kDa). For its cytotoxic action, C2II binds to a cell membrane receptor and induces cell entry of C2I via receptor-mediated endocytosis. Here we studied the structure-function relationship of C2II by constructing truncated C2II proteins and producing polyclonal antisera against selective regions of C2II. An antibody raised… CONTINUE READING

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To further define the region responsible for receptor binding , we constructed proteins with deletions in C2II ; specifically , they lacked amino acid residues 592 to 721 and the 7 C - terminal amino acid residues .
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