The C-terminal 88 amino acids of the Sendai virus P protein have multiple functions separable by mutation.

@article{Tuckis2002TheC8,
  title={The C-terminal 88 amino acids of the Sendai virus P protein have multiple functions separable by mutation.},
  author={Jeffery Tuckis and Sherin E. Smallwood and Joyce A. Feller and Sue A. Moyer},
  journal={Journal of virology},
  year={2002},
  volume={76 1},
  pages={
          68-77
        }
}
The Sendai virus P-L polymerase complex binds the NP-encapsidated nucleocapsid (NC) template through a P-NP interaction. To identify P amino acids responsible for binding we performed site-directed mutagenesis on the C-terminal 88 amino acids in the NC binding domain. The mutant P proteins expressed from plasmids were assayed for viral RNA synthesis and for various protein-protein interactions. All the mutants formed P oligomers and bound to L protein. While two mutants, JT3 and JT8, retained… CONTINUE READING