The Bowen–Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA

@inproceedings{Meyer2011TheBS,
  title={The Bowen–Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA},
  author={Bernard De Meyer and Jan Philip Wurm and Peter Koetter and Matthias S Leisegang and Valeska Schilling and Markus Buchhaupt and Martin Held and U. T. E. Bahr and Michael Karas and Alexander Heckel and Markus T Bohnsack and Jens W{\"o}hnert and Karl-Dieter Entian},
  booktitle={Nucleic acids research},
  year={2011}
}
The Nep1 (Emg1) SPOUT-class methyltransferase is an essential ribosome assembly factor and the human Bowen-Conradi syndrome (BCS) is caused by a specific Nep1(D86G) mutation. We recently showed in vitro that Methanocaldococcus jannaschii Nep1 is a sequence-specific pseudouridine-N1-methyltransferase. Here, we show that in yeast the in vivo target site for Nep1-catalyzed methylation is located within loop 35 of the 18S rRNA that contains the unique hypermodification of U1191 to 1-methyl-3-(3… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 42 REFERENCES

A fast and efficient translational control system for conditional expression of yeast genes

P. Kötter, J. E. Weigand, B. Meyer, K. D. Entian, B. Suess
  • Nucleic Acids Res.,
  • 2009
VIEW 1 EXCERPT