The Birth of Piglets from Embryos Cultured for 3 to 5 Days in a Simple Salt Solution Lacking Glucose.

@inproceedings{Kojima1996TheBO,
  title={The Birth of Piglets from Embryos Cultured for 3 to 5 Days in a Simple Salt Solution Lacking Glucose.},
  author={Toshiyuki Kojima and Yumie Zeniya and Akira Onishi},
  year={1996}
}
The aim of the present study was to assess whether or not a modified Whitten's medium lacking glucose and supplemented with 0.4% (w/v) bovine serum albumin (BSA) can sustain the viability of early-stage pig embryos during long-term culture followed by transfer to recipient gilts. Embryo collection was surgically carried out on Days 2, 3 or 4 (Day 0 = onset of estrus) by midventral incision. The 2-cell and 4-cell embryos recovered were placed in 100-μl droplets of Whitten's medium under mineral… CONTINUE READING