The Biology of CRISPR-Cas: Backward and Forward

  title={The Biology of CRISPR-Cas: Backward and Forward},
  author={Frank Hille and Hagen Richter and Shi Pey Wong and Majda Bratovi{\vc} and E. Charpentier},
  • Frank Hille, Hagen Richter, +2 authors E. Charpentier
  • Published 2018
  • Medicine, Biology
  • Cell
  • In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system against phages and other foreign genetic elements. Here, we review the biology of the diverse CRISPR-Cas systems and the major progress achieved in recent years in understanding the underlying mechanisms of the three stages of CRISPR-Cas immunity: adaptation, crRNA biogenesis, and interference. The ecology and regulation of CRISPR… CONTINUE READING

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    Publications referenced by this paper.
    A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
    • 7,513
    • Highly Influential
    • PDF
    Evolution and classification of the CRISPR–Cas systems
    • 1,575
    • PDF
    CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
    • 3,511
    • Highly Influential
    • PDF
    Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes
    • 1,622
    • PDF
    An updated evolutionary classification of CRISPR–Cas systems
    • 1,212
    • PDF
    CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
    • 1,552
    • PDF
    Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering
    • 619
    The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
    • 1,399
    • PDF
    CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA
    • 1,171
    • Highly Influential
    • PDF