The Biology of CRISPR-Cas: Backward and Forward

@article{Hille2018TheBO,
  title={The Biology of CRISPR-Cas: Backward and Forward},
  author={Frank Hille and Hagen Richter and Shi Pey Wong and Majda Bratovi{\vc} and Sarah Ressel and Emmanuelle Charpentier},
  journal={Cell},
  year={2018},
  volume={172},
  pages={1239-1259}
}

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References

SHOWING 1-10 OF 240 REFERENCES
Evolution and Ecology of CRISPR
TLDR
The current understanding of the evolutionary ecology of CRISPR-Cas systems is summarized, their value as model systems to answer fundamental questions concerning host–parasite coevolution are highlighted, and how they can be useful tools for scientists across virtually all disciplines are explained.
CRISPR–Cas systems: beyond adaptive immunity
TLDR
Recent studies that have provided insights into these unconventional CRISPR–Cas functions are discussed and their potential evolutionary implications are considered.
Unravelling the structural and mechanistic basis of CRISPR–Cas systems
TLDR
This Review summarizes the recent structural and biochemical insights that have been gained for the three major types of CRISPR–Cas systems, which together provide a detailed molecular understanding of the unique and conserved mechanisms of RNA-guided adaptive immunity in bacteria and archaea.
Inhibition of CRISPR-Cas systems by mobile genetic elements.
Regulation of CRISPR-Cas adaptive immune systems.
Evolution and classification of the CRISPR–Cas systems
TLDR
An updated analysis of the evolutionary relationships between CRISPR–Cas systems and Cas proteins is provided and a 'polythetic' classification that integrates the phylogenies of the most common cas genes, the sequence and organization of theCRISPR repeats and the architecture of the CRISpr–cas loci is proposed.
CRISPR-Cas
TLDR
The tight association of the CRISPR-Cas immunity systems with predicted toxins that, upon activation, would induce dormancy or cell death suggests that adaptive immunity and dormancy/suicide response are functionally coupled.
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
TLDR
It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
Cas9 function and host genome sampling in Type II-A CRISPR-Cas adaptation.
TLDR
Surprisingly, it is found that Cas9, previously identified as the nuclease responsible for ultimate invader destruction, is also essential for adaptation, which indicates that the process of adaptation by a Type II-A CRISPR-Cas system in Streptococcus thermophilus requires Cas1, Cas2, and Csn2.
...
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3
4
5
...