The Binding of [3H]AMPA, a Structural Analogue of Glutamic Acid, to Rat Brain Membranes

@article{Honor1982TheBO,
  title={The Binding of [3H]AMPA, a Structural Analogue of Glutamic Acid, to Rat Brain Membranes},
  author={Tage Honor{\'e} and J. B. Lauridsen and Povl Krogsgaard‐Larsen},
  journal={Journal of Neurochemistry},
  year={1982},
  volume={38}
}
Abstract: Binding of [3H]AMPA to rat brain membranes was investigated. The binding was saturable and reversible at physiological pH. Computer‐aided Scatchard analysis of the binding data, as determined by using l‐glutamic acid (l‐GLU) to define nonspecific binding, suggested the presence of two independent binding sites, with KDs of 9 and 2440 nm, respectively. Additional freezing, thawing and washing sequences gave membranes with only one binding site, with a KD of 278 nm. [3H]AMPA binding… Expand
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