The Bacillus subtilis endospore: assembly and functions of the multilayered coat

@article{McKenney2013TheBS,
  title={The Bacillus subtilis endospore: assembly and functions of the multilayered coat},
  author={Peter T. McKenney and Adam Driks and Patrick Eichenberger},
  journal={Nature Reviews Microbiology},
  year={2013},
  volume={11},
  pages={33-44}
}
Sporulation in Bacillus subtilis involves an asymmetric cell division followed by differentiation into two cell types, the endospore and the mother cell. The endospore coat is a multilayered shell that protects the bacterial genome during stress conditions and is composed of dozens of proteins. Recently, fluorescence microscopy coupled with high-resolution image analysis has been applied to the dynamic process of coat assembly and has shown that the coat is organized into at least four distinct… 
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425 Citations
Highly Influential Citations
29
Background Citations
207
Methods Citations
2
Results Citations
4

425 Citations

Shaping an Endospore: Architectural Transformations During Bacillus subtilis Sporulation.

New insights are discussed into the mechanisms that drive morphogenesis, with special emphasis on polar septation, chromosome translocation, and the phagocytosis-like process of engulfment, and also the key experimental advances that have proven valuable in revealing the inner workings of bacterial cells.

The New Kid on the Block: A Specialized Secretion System during Bacterial Sporulation.

Genetics and Biochemistry of Sporulation in Endospore-Forming Bacteria (Bacillus): A Prime Example of Developmental Biology

The present chapter encompasses a detailed description of the genes involved in the bacterial sporulation process, their location in the chromosome of Bacillus subtilis, and a descriptions of the main antibiotics produced by different Bacillus species.

Milestones in Bacillus subtilis sporulation research

This review highlights foundational discoveries that shaped the sporulation field, from its origins to the present day, tracing a chronology that spans more than one hundred eighty years.

A dynamic, ring-forming MucB / RseB-like protein influences spore shape in Bacillus subtilis

Functional diversification of the MucB / RseB protein domain between diderm and monoderm bacteria is suggested and SsdC appears to play a role in the proper assembly of the spore cortex, through connections to thespore coat.

Coordinated Assembly of the Bacillus anthracis Coat and Exosporium during Bacterial Spore Outer Layer Formation

This work dramatically improves the understanding of the assembly of the outermost layer of the B. anthracis spore, the exosporium, a layer that encases spores from many bacterial species and likely plays important roles in the spore’s interactions with the environment, including host tissues, by identifying a novel protein interaction network that directs assembly to initiate at a specific subcellular location in the developing cell.

Bacillus subtilis Spore Inner Membrane Proteome.

Over 900 proteins were identified from the B. subtilis spore IM preparations, at least some of which are likely to provide new insights into IM physiology, unveiling proteins putatively involved in spore germination machinery and hence putative germination inhibition targets.

The Direct Interaction between Two Morphogenetic Proteins Is Essential for Spore Coat Formation in Bacillus subtilis

It is reported that CotH also controls the assembly of CotE and that this mutual dependency is due to a direct interaction between the two proteins.

CotG Mediates Spore Surface Permeability in Bacillus subtilis

CotG, a modular and abundant component of the Bacillus subtilis spore coat, controls spore permeability through its central region, formed by positively charged tandem repeats, which act as spacers between the N and C termini of the protein.

Flexibility of the Prograamme of Spore Coat Formation in Bacillus subtilis: Bypass of CotE Requirement by Over-Production of CotH

Western blot, fluorescence microscopy and Surface-Enhanced Raman Scattering spectroscopy data indicate that over-production of CotH allowed the formation of spores overall resembling wild type spores and carrying in their coat some CotE−/CotH-dependant proteins.
...

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