The Bacillus subtilis AddAB helicase/nuclease is regulated by its cognate Chi sequence in vitro.

@article{Chedin2000TheBS,
  title={The Bacillus subtilis AddAB helicase/nuclease is regulated by its cognate Chi sequence in vitro.},
  author={Frederic Chedin and S. Dusko Ehrlich and Stephen C Kowalczykowski},
  journal={Journal of molecular biology},
  year={2000},
  volume={298 1},
  pages={
          7-20
        }
}
The AddAB enzyme is important to homologous DNA recombination in Bacillus subtilis, where it is thought to be the functional counterpart of the RecBCD enzyme of Escherichia coli. In vivo, AddAB responds to a specific five-nucleotide sequence (5'-AGCGG-3' or its complement) in a manner analogous to the response of the RecBCD enzyme to interaction with chi sequences. Here, we show that purified AddAB enzyme is able to load at a double-stranded DNA end and is both a DNA helicase and nuclease… CONTINUE READING
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A ®ve-nucleotide sequence protects DNA from exonucleolytic degradation by AddAB, the RecBCD analogue of Bacillus subtilis

F. CheÂdin, P. Noirot, V. Biaudet, S. D. Ehrlich
Mol. Microbiol • 1998
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