The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS).

@article{Bertrand2002TheBA,
  title={The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRS).},
  author={Lucie Bertrand and St{\'e}phane Parent and Mireille Caron and Mireille Legault and Erik Joly and St{\'e}phane Angers and Michel Bouvier and Mike S. Brown and Benoit Houle and Luc M{\'e}nard},
  journal={Journal of receptor and signal transduction research},
  year={2002},
  volume={22 1-4},
  pages={533-41}
}
In BRET2 (Bioluminescence Resonance Energy Transfer), a Renilla luciferase (RLuc) is used as the donor protein, while a Green Fluorescent Protein (GFP2) is used as the acceptor protein. In the presence of the cell permeable substrate DeepBlueC, RLuc emits blue light at 395 nm. If the GFP2 is brought into close proximity to RLuc via a specific biomolecular interaction, the GFP2 will absorb the blue light energy and reemit green light at 510nm. BRET2 signals are therefore easily determined by… CONTINUE READING

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