The Aspergillus fumigatus cellobiohydrolase B (cbhB) promoter is tightly regulated and can be exploited for controlled protein expression and RNAi.

@article{Bromley2006TheAF,
  title={The Aspergillus fumigatus cellobiohydrolase B (cbhB) promoter is tightly regulated and can be exploited for controlled protein expression and RNAi.},
  author={Michael J. Bromley and C L Gordon and N{\'u}ria Rovira-Graells and Jason Oliver},
  journal={FEMS microbiology letters},
  year={2006},
  volume={264 2},
  pages={
          246-54
        }
}
The utility of the Aspergillus fumigatus cellobiohydrolase cbhB promoter for controlled gene expression has been investigated. cbhB message was present at high levels in the presence of carboxymethylcellulose and undetected in the presence of glucose. A reporter construct using the cbhB promoter showed similar behaviour and gave lower message levels than the Aspergillus nidulans alcA promoter under repressing conditions. An RNAi construct driven by the cbhB promoter was used to down-regulate… 

Figures and Tables from this paper

T7-RNA polymerase dependent RNAi system in Aspergillus fumigatus: a proof of concept study.
TLDR
An RNAi system based on T7 RNA polymerase (TRNAP) was designed and examined in Aspergillus fumigatus and it was demonstrated that this silencing system is unstable and may not be applicable in preparation of RNAi libraries.
Evidence for Dicer-dependent RNA interference in the industrial penicillin producer Penicillium chrysogenum.
TLDR
Several lines of evidence for RNA silencing in the industrial beta-lactam antibiotic producer Penicillium chrysogenum are provided using the DsRed reporter gene under the control of the constitutive trpC promoter or the inducible xylP promoter.
Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus.
TLDR
An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus, suggesting that this approach may overcome the variable downregulation observed with integrative constructs.
High Efficiency and Stable RNA Interference Vector Construction forPenicillium sp.
TLDR
Considering the conservatism of RNAi machinery in fungi, the RNAi vector established in this study- pHX-RNAi could be applied to other filamentous fungi.
Efficiency of different strategies for gene silencing in Botrytis cinerea
TLDR
The degree of silencing achieved was high with both methods, but the hpRNA strategy resulted in a higher proportion of strongly silenced transformants, which induced a decrease in GFP levels in more than 90 % of transformants.
Essential Genes in Aspergillus fumigatus
TLDR
This chapter discusses recent developments in the identification of essential genes and validation of potential antifungal drug targets in Aspergillus fumigatus, and highlights genes involved in various biological and biochemical functions, such as amino acid, cell wall, ergosterol, heme, and lipid biosynthesis, which provide new targets for therapeutic intervention.
RNAi as a potential tool for biotechnological applications in fungi
TLDR
There is an emerging role for RNAi as a potential tool for biotechnological applications in fungi, demonstrating the contribution of RNAi to progress research and applications in biomaterials production, bioconversion, plant fungal interactions and virulence factors of human pathogens.
...
...

References

SHOWING 1-10 OF 22 REFERENCES
Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus
TLDR
The construction of plasmid vectors that can be used to regulate gene expression in A. fumigatus using a simple co-transfection approach are described and provide a new set of options to experimentally manipulate the level of specific gene products in this opportunistic mould.
GlaA Promoter Controlled Production of a Mutant Green Fluorescent Protein (S65T) by Recombinant Aspergillus niger during Growth on Defined Medium in Batch and Fed‐Batch Cultures
TLDR
The first successful expression of the Aequorea victoria green fluorescent protein (GFP) gene in Aspergillus niger is described and the expression of a mutant form of the green fluorescent proteins resulted in the formation of a functional fluorescent polypeptide.
The Developmentally Regulated alb1 Gene ofAspergillus fumigatus: Its Role in Modulation of Conidial Morphology and Virulence
TLDR
The results suggest that disruption of alb1 causes pleiotropic effects on conidial morphology and fungal virulence.
Production of recombinant proteins in the filamentous fungus Trichoderma reesei.
Gene silencing with RNA interference in the human pathogenic fungus Aspergillus fumigatus.
Aspergillus fumigatus is an opportunistic pathogenic fungus which causes fatal invasive aspergillosis among immunocompromised patients. To obtain a better understanding of the key elements involved
Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5′-decarboxylase gene, pyrG, as a unique transformation marker
TLDR
A 8.6-kb disruption cassette consisting of the Aspergillus niger pyrG gene flanked by a direct repeat that encodes the neomycin phosphotransferase of transposon Tn5 was constructed to disrupt another gene.
...
...