The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network.

@article{Chen2016TheAG,
  title={The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network.},
  author={Pei-wen Chen and Xiaoying Jian and Sarah M. Heissler and Kang Le and Ruibai Luo and Lisa M Miller Jenkins and Attila B. Nagy and Joel Moss and James R Sellers and Paul A Randazzo},
  journal={The Journal of biological chemistry},
  year={2016},
  volume={291 14},
  pages={
          7517-26
        }
}
ASAP1 regulates F-actin-based structures and functions, including focal adhesions (FAs) and circular dorsal ruffles (CDRs), cell spreading and migration. ASAP1 function requires its N-terminal BAR domain. We discovered that nonmuscle myosin 2A (NM2A) directly bound the BAR-PH tandem of ASAP1in vitro ASAP1 and NM2A co-immunoprecipitated and colocalized in cells. Knockdown of ASAP1 reduced colocalization of NM2A and F-actin in cells. Knockdown of ASAP1 or NM2A recapitulated each other's effects… CONTINUE READING
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