The Arabidopsis NRG2 Protein Mediates Nitrate Signaling and Interacts with and Regulates Key Nitrate Regulators[OPEN]

@article{Xu2016TheAN,
  title={The Arabidopsis NRG2 Protein Mediates Nitrate Signaling and Interacts with and Regulates Key Nitrate Regulators[OPEN]},
  author={Na Xu and Rongchen Wang and Lufei Zhao and Chengfei Zhang and Zehui Li and Zhao Lei and Fei Liu and Peizhu Guan and Zhaohui Chu and Nigel M. Crawford and Yong Wang},
  journal={Plant Cell},
  year={2016},
  volume={28},
  pages={485 - 504}
}
Arabidopsis NRG2, which regulates NRT1.1 and interacts with NLP7, mediates nitrate signaling. We show that NITRATE REGULATORY GENE2 (NRG2), which we identified using forward genetics, mediates nitrate signaling in Arabidopsis thaliana. A mutation in NRG2 disrupted the induction of nitrate-responsive genes after nitrate treatment by an ammonium-independent mechanism. The nitrate content in roots was lower in the mutants than in the wild type, which may have resulted from reduced expression of… Expand
The Arabidopsis NLP7 gene regulates nitrate signaling via NRT1.1–dependent pathway in the presence of ammonium
Nitrate is not only an important nutrient but also a signaling molecule for plants. A few of key molecular components involved in primary nitrate responses have been identified mainly by forward andExpand
The long noncoding RNA T5120 regulates nitrate response and assimilation in Arabidopsis.
  • Fei Liu, Yiran Xu, +7 authors Yong Wang
  • Medicine, Chemistry
  • The New phytologist
  • 2019
TLDR
A new regulatory mechanism in which lncRNA T5120 functions in nitrate regulation is revealed, providing new insights into the nitrate signaling network and can promote nitrate assimilation and plant growth to improve the nitrogen use efficiency. Expand
FIP1 Plays an Important Role in Nitrate Signaling and Regulates CIPK8 and CIPK23 Expression in Arabidopsis
TLDR
It is reported that the FIP1 gene (factor interacting with poly(A) polymerase 1) plays an important role in nitrate signaling in Arabidopsis thaliana and is interacts with the cleavage and polyadenylation specificity factor 30-L (CPSF30-L), which is also an essential player in nitrates signaling. Expand
The Arabidopsis CPSF30-L gene plays an essential role in nitrate signaling and regulates the nitrate transceptor gene NRT1.1.
TLDR
The identification of a nitrate regulatory mutant whose mutation mapped to the Cleavage and Polyadenylation Specificity Factor 30 gene (CPSF30-L) is reported, indicating that the 65 kDa protein encoded by CPSF 30-L mediates nitrate signaling in part by regulating NRT1.1 expression, thus adding an important component to the nitrate signaled network. Expand
Genome-wide analysis in response to nitrogen and carbon identifies regulators for root AtNRT2 transporters.
TLDR
An original systems biology approach is used to model a regulatory gene network targeting NRT2.5 in response to N or C signals and reveals a role for TGA3, MYC1, and bHLH093 in controlling the expression of root N RT2 transporter genes. Expand
NRT1.1 Regulates Nitrate Allocation and Cadmium Tolerance in Arabidopsis
TLDR
The results suggest that NRT1.1 regulates NO3- allocation to roots by coordinating Cd2+ accumulation in root vacuoles, which facilitates Cd1+ detoxification and reduces their cytosolic allocation and transport to the shoots. Expand
HBI Transcription Factor-Mediated ROS Homeostasis Regulates Nitrate Signal Transduction.
TLDR
It is demonstrated that HBI-mediated ROS homeostasis regulates nitrate signal transduction through modulating the nucleocytoplasmic shuttling of NLP7, thereby forming a feedback regulatory loop to regulate plant growth and development. Expand
Genome-wide Analysis In Response to N and C Identifies New Regulators for root AtNRT2 Transporters
TLDR
System analysis of the data highlighted the potential role of three putative transcription factors, TGA3, MYC1 and bHLH093, in the regulation of NRT2s transporters using a systems biology approach, and confirmed that all 3 transcription factors are regulators of N RT2.5 in response to N or C signals. Expand
Major transcription factor families involved in nitrogen stress adaptation in plants
TLDR
Nitrate (NO3−) is the major inorganic N form in aerobic soils and also a potent signal that regulates plant growth and metabolism and can be exploited for enhancing NUE and N stress adaptation in crop plants. Expand
Overexpression of the Maize ZmNLP6 and ZmNLP8 Can Complement the Arabidopsis Nitrate Regulatory Mutant nlp7 by Restoring Nitrate Signaling and Assimilation
TLDR
Nine ZmNLP genes in maize are identified and the functions of two ZMNLP members in nitrate signaling are analyzed, indicating that ZnNLP6 and ZnLP8 can replace the essential roles of the master nitrate regulatory gene AtNLP7 in nitrates signaling and metabolism. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 58 REFERENCES
A Genetic Screen for Nitrate Regulatory Mutants Captures the Nitrate Transporter Gene NRT1.11[W][OA]
TLDR
Genes involved in nitrate assimilation, energy metabolism, and pentose-phosphate pathway were most affected and strongly support the model that NRT1.1 acts as a nitrate regulator or sensor in Arabidopsis. Expand
Arabidopsis NIN-like transcription factors have a central role in nitrate signalling.
TLDR
It is shown that Arabidopsis NIN-LIKE PROTEIN (NLP) family proteins bind the nitrate-responsive cis-element and activate nitrate's responsiveness to signals, and the activity of NLPs is post-translationally modulated by nitrate signalling. Expand
The Arabidopsis Nitrate Transporter NRT1.8 Functions in Nitrate Removal from the Xylem Sap and Mediates Cadmium Tolerance[C][W]
TLDR
The functional characterization of NRT1.8, a member of the nitrate transporter (NRT1) family in Arabidopsis thaliana, suggests that NRT 1.8-regulated nitrate distribution plays an important role in Cd2+ tolerance. Expand
Transcript Profiling in the chl1-5 Mutant of Arabidopsis Reveals a Role of the Nitrate Transporter NRT1.1 in the Regulation of Another Nitrate Transporter, NRT2.1w⃞
TLDR
The results show that normal regulation of NRT2.1 expression is strongly altered in the chl1-5 mutant, where this gene is no more repressible by high N provision to the plant, and indicates that NRT1.1 plays an unexpected but important role in the regulation of both N RT2. Expand
Genomic Analysis of the Nitrate Response Using a Nitrate Reductase-Null Mutant of Arabidopsis1[w]
TLDR
A nitrate reductase (NR)-null mutant of Arabidopsis was constructed that had a deletion of the major NR gene NIA2 and an insertion in the NIA1 NR gene, and there was substantial organ specificity to the nitrate response. Expand
The nodule inception-like protein 7 modulates nitrate sensing and metabolism in Arabidopsis.
TLDR
It is reported that Arabidopsis thaliana NIN-like protein 7 (NLP7) knockout mutants constitutively show several features of nitrogen-starved plants, and that they are tolerant to drought stress. Expand
Nitrate-responsive miR393/AFB3 regulatory module controls root system architecture in Arabidopsis thaliana
TLDR
Interestingly, regulation of RSA by nitrate was specifically mediated by AFB3, indicating that miR393/AFB3 is a unique N-responsive module that controls root system architecture in response to external and internal N availability in Arabidopsis. Expand
AtCIPK8, a CBL-interacting protein kinase, regulates the low-affinity phase of the primary nitrate response.
TLDR
Results indicate that CBL-CIPK networks are responsible not only for stress responses and potassium shortage, but also for nitrate sensing. Expand
The Arabidopsis NRT1.1 transporter participates in the signaling pathway triggering root colonization of nitrate-rich patches
TLDR
It is concluded that NRT1.1, which is localized at the forefront of soil exploration by the roots, is a key component of the NO3−-sensing system that enables the plant to detect and exploit NO3-rich soil patches. Expand
Systems approaches map regulatory networks downstream of the auxin receptor AFB3 in the nitrate response of Arabidopsis thaliana roots
TLDR
It is found that the NAC4 transcription factor is a key regulatory element controlling a nitrate-responsive network, and that nac4 mutants have altered lateral root growth but normal primary root growth in response to nitrate. Expand
...
1
2
3
4
5
...