The Arabidopsis NPR1 gene confers broad-spectrum disease resistance in strawberry

  title={The Arabidopsis NPR1 gene confers broad-spectrum disease resistance in strawberry},
  author={Katchen Julliany Pereira Silva and Asha M. Brunings and Natalia A. Peres and Zhonglin Mou and Kevin M. Folta},
  journal={Transgenic Research},
Although strawberry is an economically important fruit crop worldwide, production of strawberry is limited by its susceptibility to a wide range of pathogens and the lack of major commercial cultivars with high levels of resistance to multiple pathogens. The objective of this study is to ectopically express the Arabidopsis thalianaNPR1 gene (AtNPR1) in the diploid strawberry Fragaria vesca L. and to test transgenic plants for disease resistance. AtNPR1 is a key positive regulator of the long… 

Transgenic Citrus Expressing an Arabidopsis NPR1 Gene Exhibit Enhanced Resistance against Huanglongbing (HLB; Citrus Greening)

Overexpression of AtNPR1 resulted in trees with normal phenotypes that exhibited enhanced resistance to HLB and a few lines remained disease-free even after 36 months of planting in a high-disease pressure field site.

NPR1 as a transgenic crop protection strategy in horticultural species

The progress on the understanding of NPR1-centered applications in horticultural and other crop plants is summarized and the future challenges of using NPR1 in agricultural applications are outlined.

Bacterial resistance in AtNPR1 transgenic sweet orange is mediated by priming and involves EDS1 and PR2

The use of AtNPR1 is an alternative against citrus canker since it led to higher tolerance in a pathogen-dependent response and the stronger expression of EDS1 and PR2 after pathogen inoculation correlated with a higher tolerance response suggesting a possible priming effect.

Knockout of SlNPR1 enhances tomato plants resistance against Botrytis cinerea by modulating ROS homeostasis and JA/ET signaling pathways.

It is revealed that knockout of SlNPR1 resulted in increased activities of defense enzymes, changes in ROS homeostasis and integrity of cell walls, and activation of JA and ET pathways, which confers resistance against B. cinerea in tomato plants.

Overexpression of the Arabidopsis NPR1 protein in citrus confers tolerance to Huanglongbing

Results demonstrate that overexpression of the AtNPR1 protein in citrus is able to provide robust tolerance to HLB, and find that the same three transgenic lines are resistant to citrus canker.

Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

In our earlier investigation, we had demonstrated that transgenic cotton plants expressing AtNPR1 showed significant tolerance to Fusarium oxysporum f. sp. vasinfectum, isolate 11 (Fov11) and several

Characterization of NPR1 and NPR4 genes from mulberry (Morus multicaulis) and their roles in development and stress resistance.

The data suggest that both the MuNPR1 and MuNPR4 genes play a role in the coordination between signaling pathways, and the information provided here enables the in-depth functional analysis of the Mu NPR1 andMu NPR4 genes and may promote mulberry resistance breeding in the future.

The History and Current Status of Genetic Transformation in Berry Crops

The recalcitrance of Rubus tissues to regenerate in vitro has impeded the development of robust transformation protocols in these species, although a few number of studies have successfully obtained transgenic plants carrying genes of interest.

Cloning and characterization of a Mimulus lewisii NPR1 gene involved in regulating plant resistance to Rhizoctonia solani.

The present study suggests that increasing the expression level of MlNPR1 may be a promising approach for development of monkey flower cultivars with enhanced resistance to diseases.

Tools for engineering resistance against pathogens in plants

The most recent strategies that have been utilized for exploiting the host and pathogen genes for disease resistance in plants are summarized.



Transgenic tomato plants expressing the Arabidopsis NPR1 gene display enhanced resistance to a spectrum of fungal and bacterial diseases

The potential of using SAR-related genes for the genetic engineering of enhanced resistance to multiple diseases in tomato is explored, and transgenic lines that accumulated higher levels of NPR1 proteins exhibited higher levels and a broader spectrum ofEnhanced resistance to the diseases, and enhanced disease-resistance was stably inherited.

Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1.

It is suggested that FHB resistance in the AtNPR1-expressing wheat is a result of these plants being more responsive to an endogenous activator of plant defense, and it is demonstrated that NPR1 is an effective candidate for controlling FHB.

Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light.

Results indicate that NH1 may be involved in the regulation of SA in response to environmental changes, and indicates that rice has a disease-resistance pathway similar to the Arabidopsis SAR pathway.

Constitutive expression of Arabidopsis NPR1 confers enhanced resistance to the early instars of Spodoptera litura in transgenic tobacco.

It is shown that heterologous expression of AtNPR1 provides enhanced resistance to early larval populations of the herbivore, Spodoptera in transgenic tobacco plants.

Overexpression of the apple MpNPR1 gene confers increased disease resistance in Malus x domestica.

Some MpNPR-overexpressing Galaxy lines exhibited increased resistance to two important fungal pathogens of apple, Venturia inaequalis and Gymnosporangium juniperi-virginianae.

Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis.

This is the first demonstration that the Arabidopsis NPR1 gene can enhance disease resistance in a monocot plant and suggests that monocots and dicot plants share a conserved signal transduction pathway controlling NPR1-mediated resistance.

Broad-spectrum disease resistance to necrotrophic and biotrophic pathogens in transgenic carrots (Daucus carota L.) expressing an Arabidopsis NPR1 gene

The overexpression of the SAR controlling master switch in carrot tissues offers the ability to control a wide range of different pathogens, for which there is currently little genetic resistance available.

Over-expression of the Arabidopsis NPR1 gene in citrus increases resistance to citrus canker

Over-expression of At NPR1 in citrus increases resistance to citrus canker and that the resistance is related with the expression levels of AtNPR1 in the transgenic plants, indicating a promising approach for development of more resistant cultivars to citrusCanker.

Overexpression of (At)NPR1 in rice leads to a BTH- and environment-induced lesion-mimic/cell death phenotype.

It is demonstrated that overexpression of (At)NPR1 in rice also triggers a lesion-mimic/cell death (LMD) phenotype, suggesting that (At), the central regulator of SAR in Arabidopsis, has multiple roles in plant stress responses that may affect its efficacy as a transgenic tool for engineering broad-spectrum resistance.

Functional analysis of rice NPR1-like genes reveals that OsNPR1/NH1 is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility.

This study demonstrates that rice has evolved an SA-mediated systemic acquired resistance similar to that in Arabidopsis, and also provides a practical approach for the improvement of disease resistance without the penalty of decreased herbivore resistance in rice.