Gene polymorphisms of tumor necrosis factor alpha and antioxidant enzymes in bronchial asthma.
BACKGROUND We aimed to investigate the possible association between Mn-SOD polymorphism in the mitochondrial targeting sequence and asthma. METHODS Alanine or valine polymorphism in the signal peptide of Mn-SOD gene was evaluated using a primer pair to amplify a 107-bp fragment followed by digestion with NgoM IV. RESULTS No significant difference in genotype frequencies was found between patients and controls. CONCLUSION These results suggest no major modifying role for the Mn-SOD gene polymorphism in patients with asthma.