• Corpus ID: 49389445

The Absence of CpG sequences in Plasmid DNA-Chitosan Polyplexes Increases Transfection Efficiencies and Reduces Inflammatory Responses in Murine Lungs

@inproceedings{Wongrakpanich2014TheAO,
  title={The Absence of CpG sequences in Plasmid DNA-Chitosan Polyplexes Increases Transfection Efficiencies and Reduces Inflammatory Responses in Murine Lungs},
  author={Amaraporn Wongrakpanich and Andrea Adamcakova-Dodd and Vijaya Bharti Joshi and Kranti A Mapuskar and Sean M. Geary and Douglas R. Spitz and Peter S. Thorne and Aliasger K. Salem},
  year={2014}
}
SUMMARY Chitosan polyplexes containing plasmid DNA (pDNA) have strong potential for pulmonary gene delivery applications. However, before using chitosan/pDNA polyplexes (CSpp) in clinical applications, their potential cytotoxicity needs to be investigated. We formulated CSpp in the size range from 200 to 400 nm with amine to phosphate (N/P) ratios that ranged from 1 100. We compared two types of plasmid DNA within the polyplexes; pDNA that was free of CpG sequences (CpG(-)) and pDNA that… 

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  • 2075-80. ACKNOWLEDGMENTS The authors gratefully acknowledge support from the Environmental Health Sciences Research Center