The Ability of a Recombinant Escherichia coli Strain to Synthesize 2-C-Methyl-D-Erythritol-2,4-Cyclopyrophosphate Correlates with Its Tolerance to In Vitro Induced Oxidative Stress and to the Bactericidal Action of Murine Peritoneal Macrophages

@article{Ogrel1996TheAO,
  title={The Ability of a Recombinant Escherichia coli Strain to Synthesize 2-C-Methyl-D-Erythritol-2,4-Cyclopyrophosphate Correlates with Its Tolerance to In Vitro Induced Oxidative Stress and to the Bactericidal Action of Murine Peritoneal Macrophages},
  author={O. Ogrel and K. Fegeding and E. Kharatian and A. Sudarikov and D. Ostrovsky},
  journal={Current Microbiology},
  year={1996},
  volume={32},
  pages={225-228}
}
Abstract. A number of bacteria are able to synthesize 2-C-methyl-D-erythritol-2,4-cyclopyrophosphate (BOSS) in response to oxidative stress. Here we show that the ability to synthesize BOSS can be genetically transferred from Corynebacterium ammoniagenes to Escherichia coli. A total DNA library from C. ammoniagenes ATCC 6872 established in the pBluescript SKII+ vector backbone was transfected into E. coli XL-1 blue. Recombinant clone 2–31, which was resistant to redox-cycling agents, was… Expand
6 Citations
Combinatorial Engineering of 1-Deoxy-D-Xylulose 5-Phosphate Pathway Using Cross-Lapping In Vitro Assembly (CLIVA) Method
TLDR
This study demonstrated the utility of CLIVA in the assembly of multiple fragments of DNA into a plasmid which enabled the rapid exploration of biological pathways and revealed the unanticipated inhibitory effects of certain combinations of genes on the production of amorphadiene. Expand
2C-Methyl-d-erythritol 4-phosphate enhances and sustains cyclodiphosphate synthase IspF activity.
TLDR
The results suggest a novel feed-forward regulatory mechanism for 2C-methyl-d-erythritol 2,4-cyclodiphosphate (MEcDP) production and support an isoprenoid biosynthesis regulatory mechanism via feedback inhibition of the IspF-MEP complex by FDP. Expand
BIOSYNTHESIS OF 2-C-METHYL-D-ERYTHRITOL IN PLANTS BY REARRANGEMENT OF THE TERPENOID PRECURSOR, 1-DEOXY-D-XYLULOSE 5-PHOSPHATE
Leaves of Liriodendron tulipifera convert 1-deoxy-D-xylulose to 2-C-methyl-D-erythritol via a skeletal rearrangement reminiscent of the formation of terpene precursors from 1-deoxy-D-xylulose. TheExpand
Synthesis of enantiopure 2-C-methyl-D-erythritol 4-phosphate and 2,4-cyclodiphosphate from D-arabitol.
TLDR
Two key intermediates of the newly discovered mevalonate-independent pathway for isoprenoid biosynthesis were prepared and offers a straightforward route to analogues and labeled forms. Expand
2-C-methylerythritol phosphate pathway of isoprenoid biosynthesis as a target in identifying new antibiotics, herbicides, and immunomodulators: A review
  • Y. Ershov
  • Biology, Medicine
  • Applied Biochemistry and Microbiology
  • 2007
Specific inhibitors of 2-C-methylerythritol phosphate pathway (MEP-pathway), including compounds obtained based on its metabolites, may compose a new class of antibiotics combining high efficiencyExpand
The deoxyxylulose phosphate pathway of terpenoid biosynthesis in plants and microorganisms.
Recent studies have uncovered the existence of an alternative, non-mevalonate pathway for the formation of isopentenyl pyrophosphate and dimethylallyl pyrophosphate, the two building blocks ofExpand

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