The Ability of a Recombinant Escherichia coli Strain to Synthesize 2-C-Methyl-D-Erythritol-2,4-Cyclopyrophosphate Correlates with Its Tolerance to In Vitro Induced Oxidative Stress and to the Bactericidal Action of Murine Peritoneal Macrophages

  title={The Ability of a Recombinant Escherichia coli Strain to Synthesize 2-C-Methyl-D-Erythritol-2,4-Cyclopyrophosphate Correlates with Its Tolerance to In Vitro Induced Oxidative Stress and to the Bactericidal Action of Murine Peritoneal Macrophages},
  author={O. Ogrel and K. Fegeding and E. Kharatian and A. Sudarikov and D. Ostrovsky},
  journal={Current Microbiology},
Abstract. A number of bacteria are able to synthesize 2-C-methyl-D-erythritol-2,4-cyclopyrophosphate (BOSS) in response to oxidative stress. Here we show that the ability to synthesize BOSS can be genetically transferred from Corynebacterium ammoniagenes to Escherichia coli. A total DNA library from C. ammoniagenes ATCC 6872 established in the pBluescript SKII+ vector backbone was transfected into E. coli XL-1 blue. Recombinant clone 2–31, which was resistant to redox-cycling agents, was… Expand
6 Citations
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2C-Methyl-d-erythritol 4-phosphate enhances and sustains cyclodiphosphate synthase IspF activity.
The results suggest a novel feed-forward regulatory mechanism for 2C-methyl-d-erythritol 2,4-cyclodiphosphate (MEcDP) production and support an isoprenoid biosynthesis regulatory mechanism via feedback inhibition of the IspF-MEP complex by FDP. Expand
Leaves of Liriodendron tulipifera convert 1-deoxy-D-xylulose to 2-C-methyl-D-erythritol via a skeletal rearrangement reminiscent of the formation of terpene precursors from 1-deoxy-D-xylulose. TheExpand
Synthesis of enantiopure 2-C-methyl-D-erythritol 4-phosphate and 2,4-cyclodiphosphate from D-arabitol.
Two key intermediates of the newly discovered mevalonate-independent pathway for isoprenoid biosynthesis were prepared and offers a straightforward route to analogues and labeled forms. Expand
2-C-methylerythritol phosphate pathway of isoprenoid biosynthesis as a target in identifying new antibiotics, herbicides, and immunomodulators: A review
  • Y. Ershov
  • Biology, Medicine
  • Applied Biochemistry and Microbiology
  • 2007
Specific inhibitors of 2-C-methylerythritol phosphate pathway (MEP-pathway), including compounds obtained based on its metabolites, may compose a new class of antibiotics combining high efficiencyExpand
The deoxyxylulose phosphate pathway of terpenoid biosynthesis in plants and microorganisms.
Recent studies have uncovered the existence of an alternative, non-mevalonate pathway for the formation of isopentenyl pyrophosphate and dimethylallyl pyrophosphate, the two building blocks ofExpand


The ability of bacteria to synthesize a new cyclopyrophosphate correlates with their tolerance to redox-cycling drugs: on a crossroad of chemotherapy, environmental toxicology and immunobiochemical problems.
Many redox-cyclers were recently shown to induce, in some bacterial species, large-scale biosynthesis of a new 2-methylbutan-1,2,3,4-tetraol-2,4-cyclopyrophosphate believed to be involved inExpand
Activation by nitric oxide of an oxidative-stress response that defends Escherichia coli against activated macrophages.
It is demonstrated that SoxR is a sensor for cellular exposure to NO, and that the soxRS response system may contribute to bacterial virulence. Expand
Bacteria and pesticides: a new aspect of interaction--involvement of a new biofactor.
Positively charged hydrophobic pesticides of the dipyridyl family [diquat, paraquat, benzylviologen (BV++), etc.] were shown to provoke accumulation of 2-methylbutane-1,2,3,4-tetraol-2,4-Expand
Natural and synthetic non-peptide antigens recognized by human γδ T cells
Results provide formal evidence that, in contrast to recognition of major histocompatibility complex-bound peptide antigens by αβ T cells, human γδ T cells can recognize naturally occurring small non-peptidic antIGens. Expand
Phosphate is essential for stimulation of Vγ9Vδ2 T lymphocytes by mycobacterial low molecular weight ligand
T lymphocytes are divided into two subsets which express different T cell receptor heterodimers. In the peripheral blood of healthy individuals, the majority of T cells express the α/β T cellExpand
Stimulation of human gamma delta T cells by nonpeptidic mycobacterial ligands.
Findings support the hypothesis that some gamma delta T cells recognize nonpeptidic ligands from Mycobacterium tuberculosis strain H37Rv, which is a 5' triphosphorylated thymidine-containing compound, to which the three other stimulatory molecules are structurally related. Expand
The ability of bacteria to accumulate diglycosylpyrophosphate is induced by oxidative stress
  • 1991
Synthesis of 2-C-methylD-erythritol2,4-cyclopyrophosphate in recombinant culture of Escherichia coli
  • 1995
Stimulation of human g / d T cells by nonpeptidic Mycobacterial ligands
  • Science
  • 1994
Bacterial oxidative-stress substance is 2-C-methyl-D-erythritol 2,4-cyclopyrophosphate.