The ATPase domain of ISWI is an autonomous nucleosome remodeling machine

@article{MuellerPlanitz2013TheAD,
  title={The ATPase domain of ISWI is an autonomous nucleosome remodeling machine},
  author={Felix Mueller-Planitz and Henrike Klinker and Johanna Ludwigsen and Peter B. Becker},
  journal={Nature Structural &Molecular Biology},
  year={2013},
  volume={20},
  pages={82-89}
}
ISWI slides nucleosomes along DNA, enabling the structural changes of chromatin required for the regulated use of eukaryotic genomes. Prominent mechanistic models imply cooperation of the ISWI ATPase domain with a C-terminal DNA-binding function residing in the HAND-SANT-SLIDE (HSS) domain. Contrary to these models, we show by quantitative biochemical means that all fundamental aspects of nucleosome remodeling are contained within the compact ATPase module of Drosophila ISWI. This domain can… CONTINUE READING