The ATPase cycle of the mitochondrial Hsp90 analog Trap1.

  title={The ATPase cycle of the mitochondrial Hsp90 analog Trap1.},
  author={Adriane Leskovar and Harald Wegele and Nicolas D. Werbeck and Johannes Buchner and Jochen Reinstein},
  journal={The Journal of biological chemistry},
  volume={283 17},
Hsp90 is an ATP-dependent molecular chaperone whose mechanism is not yet understood in detail. Here, we present the first ATPase cycle for the mitochondrial member of the Hsp90 family called Trap1 (tumor necrosis factor receptor-associated protein 1). Using biochemical, thermodynamic, and rapid kinetic methods we dissected the kinetics of the nucleotide-regulated rearrangements between the open and the closed conformations. Surprisingly, upon ATP binding, Trap1 shifts predominantly to the… CONTINUE READING