The AKR thymoma BW5147 is able to produce lymphokines when stimulated with calcium ionophore and phorbol ester.

@article{Hagiwara1988TheAT,
  title={The AKR thymoma BW5147 is able to produce lymphokines when stimulated with calcium ionophore and phorbol ester.},
  author={Hisashi Hagiwara and Takafumi Yokota and Jeanne Luh and Fan Lee and Ken-ichi Arai and Naoko Arai and Albert Zlotnik},
  journal={Journal of immunology},
  year={1988},
  volume={140 5},
  pages={1561-5}
}
We produced the T cell hybridoma D9C1.12.17 by fusing an IL-4-producing T cell clone D9.1Hi with the AKR thymoma BW5147. The resulting hybridoma produced IL-2 as well as IL-4 even though none of the parental cells produced IL-2 after stimulation with Con A. The production of IL-2 was confirmed at the mRNA level by using an S1 nuclease protection assay… CONTINUE READING