The A‐ and B‐type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.

  title={The A‐ and B‐type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.},
  author={Jeremy Minshull and Roy M. Golsteyn and Caroline S. Hill and Timothy Hunt},
  journal={The EMBO Journal},
Cyclins play a key role in the induction of mitosis. In this paper we report the isolation of a cyclin A cDNA clone from Xenopus eggs. Its cognate mRNA encodes a protein that shows characteristic accumulation and destruction during mitotic cell cycles. The cyclin A polypeptide is associated with a protein that cross‐reacts with an antibody against the conserved ‘PSTAIR’ epitope of p34cdc2, and the cyclin A‐cdc2 complex exhibits protein kinase activity that oscillates with the cell cycle. This… Expand
Cyclins A and B1 in the human cell cycle.
It is concluded that cyclins A and B1 may differentially regulate the cell cycle in several ways: they form complexes with distinct protein kinases and these complexes are active at different times in the cell Cycle; they form distinct multiprotein complexes, such as with the transcription factor E2F; and they are localized to different parts of the cell where different substrates will be available to them. Expand
Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells
It is shown that cyclin B2 is cytoplasmic during interphase of the cell cycle, but undergoes an abrupt translocation to the cell nucleus at the onset of mitotic prophase, and the phenotypic consequences of expressing wild-type and mutated versions of avian cyclinB2 in HeLa cells are examined. Expand
Activation of the Xenopus cyclin degradation machinery by full-length cyclin A.
The results presented here indicate thatcyclin A has not lost the ability to activate its own destruction and that cyclin A-mediated activation of the cyclin destruction pathway permitted destruction of cyclin B1 as well as cyclinA, indicating that there are not distinct Cyclin A and Cyclin B destruction pathways. Expand
Control of microtubule dynamics and length by cyclin A- and cyclin B- dependent kinases in Xenopus egg extracts
Using a simple mathematical model, it is shown how the length distributions of centrosome-nucleated microtubules relate to the four parameters that describe microtubule dynamics, which implies that well defined steady-state length distributions can be produced by regulating precisely the respective values of the dynamical parameters. Expand
Identification of a novel vertebrate cyclin: cyclin B3 shares properties with both A‐ and B‐type cyclins.
It is concluded that cyclin B3 may share functional properties with both A‐ and B‐type cyclins, reminiscent of recent results obtained with cyclin A. Expand
Role for cyclin A in the dependence of mitosis on completion of DMA replication
This data suggests that cyclin A has a distinct role in cell-cycle progression, and an antisense oligodeoxy-nucleotide directed against Cyclin A was used to investigate this role. Expand
Cyclin A in cell cycle control and cancer
Consistent with its role as a key cell cycle regulator, expression of cyclin A is found to be elevated in a variety of tumors. Expand
Cyclin A potentiates maturation-promoting factor activation in the early Xenopus embryo via inhibition of the tyrosine kinase that phosphorylates cdc2
It is proposed that the active cyclin A-cdc2 kinase generated without a lag phase from neo-synthesizedcyclin A and cdc2 may cause a rapid switch in the equilibrium of cyclin B-CDc2 complexes to the tyrosine-dephosphorylated and active form of c dc2 during early development, owing to strong inhibition of the cDC2- specific tyrosin kinase(s). Expand
Activation of p34cdc2 kinase by cyclin A
The results define a system for investigating the biochemistry and regulation of cdc2 kinase activation by cyclin A, which inhibits the degradation of both A- and B-type endogenous Xenopus cyclins. Expand
Interaction of Xenopus Cdc2·Cyclin A1 with the Origin Recognition Complex*
The results indicate that Xenopus ORC and Cdc2·cyclin A1 physically interact and demonstrate a physical link between an active cyclin-dependent kinase and proteins involved in the initiation of DNA replication. Expand


Histone Phosphorylation. Chromosomes and Chromatin
  • Trends Genet.,
  • 1988
Histone Genes
  • 1984
Protein phosphorylation.
When reading has become a habit, you will not make it as disturbing activities or as boring activity, and you can gain many benefits and importances of reading. Expand
Note added in proof The sequence reported here has been submitted to the EMBL database with the accession number X53745, and the title X.laevis cyclin A cDNA