The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.

  title={The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.},
  author={A. Nomoto and Y F Lee and Eckard J Wimmer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={73 2},
Poliovirus was grown in HeLa cells in the presence of phosphorus-32 and actinomycin D. Three to four hours after infection, viral mRNA was recovered from polyribosomes and its identity verified by two-dimensional gel electrophoresis of RNase T1 digests. Digestion of the viral [32P]mRNA with RNase T2 and separation of the products by ion exchange chromatography at pH 5 yielded pUp as possible 5' terminus but no "capping group" of the structure m7G(5')ppp(5')Np. Total cytoplasmic [32P]RNA of HeLa… CONTINUE READING


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