The 4D nucleome: Evidence for a dynamic nuclear landscape based on co‐aligned active and inactive nuclear compartments

@article{Cremer2015The4N,
  title={The 4D nucleome: Evidence for a dynamic nuclear landscape based on co‐aligned active and inactive nuclear compartments},
  author={Thomas Cremer and Marion Cremer and Barbara H{\"u}bner and Hilmar Strickfaden and Daniel Smeets and Jens Popken and Michael Sterr and Yolanda Markaki and Karsten Rippe and Christoph Cremer},
  journal={FEBS Letters},
  year={2015},
  volume={589}
}

The Interchromatin Compartment Participates in the Structural and Functional Organization of the Cell Nucleus

The role of the interchromatin compartment (IC) in shaping nuclear landscapes is focused on and it is postulated that it provides routes for imported transcription factors to target sites, for export routes of mRNA as ribonucleoproteins toward NPCs, as well as for the intranuclear passage of regulatory RNAs from sites of transcription to remote functional sites.

Nuclear compartmentalization, dynamics, and function of regulatory DNA sequences

A multilayered shell‐like chromatin organization of chromatin domain clusters with increasing chromatin compaction levels from the periphery toward the interior with a decondensed transcriptionally active peripheral layer and compact repressed chromatin typically located in the interior is suggested.

Initial high-resolution microscopic mapping of active and inactive regulatory sequences proves non-random 3D arrangements in chromatin domain clusters

The finding of a structural organization of CDCs based on radially arranged layers of different chromatin compaction levels indicates a complex higher-order chromatin organization beyond a dichotomic classification of chromatin into an ‘open,” active and ‘closed,’ inactive state.

Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments

The findings substantiate the conservation of the recently published ANC-INC network model of mammalian nuclear organization during human myelopoiesis irrespective of profound changes of the global nuclear architecture observed during this differentiation process.

Cohesin depleted cells rebuild functional nuclear compartments after endomitosis

Using live-cell and super-resolved 3D microscopy, it is demonstrated that cohesin depleted cells pass through an endomitosis and rebuild a single multilobulated nucleus (MLN) with chromosome territories (CTs) pervaded by interchromatin channels.

Live imaging of chromatin distribution reveals novel principles of nuclear architecture and chromatin compartmentalization

Physical modeling suggests that binding of lamina-associated domains combined with chromatin self-attractive interactions recapitulate the experimental chromatin distribution profiles and reveals a novel mode of mesoscale organization of peripheral chromatin sensitive to lamina composition, which is evolutionary conserved.

DNA sequence-dependent chromatin architecture and nuclear hubs formation

It is conjecture that dynamic DNA binding affinity and flexibility underlay the emergence of chromatin condensates, their growth is likely promoted in mechanically soft regions (GC-rich) of the lowest chromatin and nucleosome densities.

The 4D Nucleome: Genome Compartmentalization in an Evolutionary Context

The conservation of fundamental features of higher order chromatin arrangements throughout the evolution of metazoan animals suggests the existence of conserved, but still unknown mechanism(s) controlling this architecture.

Live imaging of chromatin distribution in muscle nuclei reveals novel principles of nuclear architecture and chromatin compartmentalization

A novel mode of mesoscale organization of chromatin within the nucleus in a live organism, in which the chromatin forms a peripheral layer separated from the nuclear interior, may be essential for robust transcriptional regulation in fully differentiated cells.
...

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