The 32P-postlabeling assay for DNA adducts

  title={The 32P-postlabeling assay for DNA adducts},
  author={David H Phillips and Volker M Arlt},
  journal={Nature Protocols},
32P-postlabeling analysis is an ultrasensitive method for the detection and quantitation of carcinogen–DNA adducts. It consists of four principal steps: (i) enzymatic digestion of DNA to nucleoside 3′-monophosphates; (ii) enrichment of the adduct fraction of the DNA digest; (iii) 5′-labeling of the adducts by transfer of 32P-orthophosphate from [γ-32P]ATP mediated by polynucleotide kinase (PNK); (iv) chromatographic or electrophoretic separation of the labeled adducts or modified nucleotides… CONTINUE READING


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