The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.

@article{Shine1974The3S,
  title={The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.},
  author={John Shine and Lynn Dalgarno},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1974},
  volume={71 4},
  pages={
          1342-6
        }
}
  • J. Shine, L. Dalgarno
  • Published 1974
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
With a stepwise degradation and terminal labeling procedure the 3'-terminal sequence of E. coli 16S ribosomal RNA is shown to be Pyd-A-C-C-U-C-C-U-U-A(OH). It is suggested that this region of the RNA is able to interact with mRNA and that the 3'-terminal U-U-A(OH) is involved in the termination of protein synthesis through base-pairing with terminator codons. The sequence A-C-C-U-C-C could recognize a conserved sequence found in the ribosome binding sites of various coliphage mRNAs; it may thus… Expand
Nucleotide sequence of a region in 23-S RNA adjacent to peptidyl transferase catalytic center of Escherichia coli ribosomes.
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Intact 3′ end of 16S rRNA is not required for specific mRNA binding
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Nucleotide sequence of the 3′ terminus of E. coli 16S ribosomal RNA
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Sequence analysis of the 3′‐T1 oligonucleotide of 16S ribsomal RNA from Escherichia coli
TLDR
The use of U2 ribonuclease and venom phosphodiesterase allowed us to deduce the sequence A-U-C-A-C/C-C+C- U-C’s/C+U-A0 ~1, which confirms the partial sequence proposed by Shine and Dalgarno [4]. Expand
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  • J. Steitz, K. Jakes
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1975
TLDR
Electrophoresis on 9% polyacrylamide gels reveals a dissociable complex containing a pure RNA-RNA hybird that strongly supports the hypothesis that during the initiation step of protein biosynthesis the 3' end of 16S RNA base pairs with the polypurine stretch common to initiator regions in E. coli and bacteriophage mRNAs. Expand
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  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1988
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3'‐terminal nucleotide sequence of Crithidia fasciculata small ribosomal subunit RNA
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This work has shown that the primary sequence at the 3’end of SSU RNA is highly conserved in both prokaryotes and eukaryotes, and argues that this remarkable preservation of primary sequence, secondary structure, and posttranscriptional modification argues that it has a crucial (and probably universal) role in protein biosynthesis. Expand
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