The 3'-untranslated region of mouse myelin basic protein gene increases the amount of mRNA in immortalized mouse oligodendrocytes.

@article{Ueno1994The3R,
  title={The 3'-untranslated region of mouse myelin basic protein gene increases the amount of mRNA in immortalized mouse oligodendrocytes.},
  author={Shigeru Ueno and Yoshikazu Kotani and Kei Kondoh and Akiko Sano and Yasuo Kakimoto and Anthony T. Campagnoni},
  journal={Biochemical and biophysical research communications},
  year={1994},
  volume={204 3},
  pages={1352-7}
}
The 3'-untranslated region (UTR) of the myelin basic protein (MBP) mRNA has been found previously to enhance the translational efficiency of the coding region by two-fold in cell-free translational systems. In this study, we transfected eukaryotic expression vectors containing the reporter cDNA for chloramphenicol acetyltransferase (CAT) with or without the mouse MBP cDNA 3'-UTR into cultured cells. CAT activity in the mouse oligodendrocyte cell line, N20.1, transfected with a CAT cDNA… CONTINUE READING