The 2-aminoethylphosphonate-specific transaminase of the 2-aminoethylphosphonate degradation pathway.

@article{Kim2002The2T,
  title={The 2-aminoethylphosphonate-specific transaminase of the 2-aminoethylphosphonate degradation pathway.},
  author={Alexander D Kim and Angela S Baker and Debra Dunaway-Mariano and William W Metcalf and Barry L. Wanner and Brian M. Martin},
  journal={Journal of bacteriology},
  year={2002},
  volume={184 15},
  pages={4134-40}
}
The 2-aminoethylphosphonate transaminase (AEPT; the phnW gene product) of the Salmonella enterica serovar Typhimurium 2-aminoethylphosphonate (AEP) degradation pathway catalyzes the reversible reaction of AEP and pyruvate to form phosphonoacetaldehyde (P-Ald) and L-alanine (L-Ala). Here, we describe the purification and characterization of recombinant AEPT. pH rate profiles (log V(m) and log V(m)/K(m) versus pH) revealed a pH optimum of 8.5. At pH 8.5, K(eq) is equal to 0.5 and the k(cat… CONTINUE READING

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