The 19-kDal protein encoded by early region 1b of adenovirus type 12 is synthesized efficiently in Escherichia coli only as a fused protein.

@article{Fukui1983The1P,
  title={The 19-kDal protein encoded by early region 1b of adenovirus type 12 is synthesized efficiently in Escherichia coli only as a fused protein.},
  author={Yuka Fukui and Izumu Saito and Kazuko Shiroki and H Shimojo and Yutaka Takebe and Yoshito Kaziro},
  journal={Gene},
  year={1983},
  volume={23 1},
  pages={1-13}
}
We have constructed recombinant plasmids that direct the synthesis of the Mr 19 000 protein encoded by the adenovirus type 12 (Ad12) E1b region as either a native protein or a protein fused to the amino-terminal portion of the elongation factor EF-TuB in Escherichia coli cells. Using these recombinants, we could synthesize a large amount of the fused protein, while only a small amount of the native Mr 19 000 protein was produced. The failure to synthesize the native Mr 19 000 protein in E. coli… CONTINUE READING

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