The 1.2-Megabase Genome Sequence of Mimivirus

@article{Raoult2004The1G,
  title={The 1.2-Megabase Genome Sequence of Mimivirus},
  author={Didier Raoult and St{\'e}phane Audic and Catherine Robert and Chantal Abergel and Patricia Renesto and Hiroyuki Ogata and Bernard La Scola and Marie Suzan and Jean-Michel Claverie},
  journal={Science},
  year={2004},
  volume={306},
  pages={1344 - 1350}
}
We recently reported the discovery and preliminary characterization of Mimivirus, the largest known virus, with a 400-nanometer particle size comparable to mycoplasma. Mimivirus is a double-stranded DNA virus growing in amoebae. We now present its 1,181,404–base pair genome sequence, consisting of 1262 putative open reading frames, 10% of which exhibit a similarity to proteins of known functions. In addition to exceptional genome size, Mimivirus exhibits many features that distinguish it from… 
Response to Comment on "The 1.2-Megabase Genome Sequence of Mimivirus"
TLDR
The size and complexity of the Mimivirus genome challenge the established frontier between viruses and parasitic cellular organisms and this new sequence data might help shed a new light on the origin of DNA viruses and their role in the early evolution of eukaryotes.
Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae
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Megavirus chilensis is presented, a giant virus isolated off the coast of Chile, but capable of replicating in fresh water acanthamoeba and exhibits three additional aminoacyl-tRNA synthetase genes adding strong support to the previous suggestion that the Mimivirus/Megav virus lineage evolved from an ancestral cellular genome by reductive evolution.
Mimivirus TyrRS: preliminary structural and functional characterization of the first amino-acyl tRNA synthetase found in a virus.
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Experimental evidence that Mimivirus tyrosyl tRNA synthetase recombinant gene product does indeed activate tyrosine is presented, and preliminary phasing was obtained using an original combination of homology modelling and normal mode analysis.
Mimivirus gene promoters exhibit an unprecedented conservation among all eukaryotes.
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An exhaustive in silico analysis of the noncoding moiety of all known viral genomes now uncovers the unprecedented perfect conservation of an AAAATTGA motif in close to 50% of the Mimivirus genes.
Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere
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Tupanvirus is a unique giant virus that has an unusually long tail and contains the largest translational apparatus of the known virosphere and their genetic analysis can provide insights into virus evolution.
The Mimivirus 1.2 Mb dsDNA genome is elegantly organized into a nuclear-like weapon
TLDR
It is shown that opening of its large icosahedral capsid in vitro leads to the release of electron dense, 30 nm diameter rod-shaped objects that appear to be expelled from the particles and unwinding in the host cytoplasm.
Mimivirus Giant Particles Incorporate a Large Fraction of Anonymous and Unique Gene Products
TLDR
The composition of purified isolated virions was analyzed by using a combined electrophoresis/mass spectrometry approach allowing the identification of 114 proteins, which encode bona fide proteins that are likely to participate in well-integrated processes.
The polyadenylation site of Mimivirus transcripts obeys a stringent 'hairpin rule'.
TLDR
Surprisingly, Mimivirus tRNAs are expressed as polyadenylated messengers, including an ORF/tRNA composite mRNA, raising once more the question of Mimvirus's evolutionary origin.
A new example of viral intein in Mimivirus
TLDR
The intriguing association of an extremophilic archaeal-type intein with a mesophilic eukaryotic-like PolB in Mimivirus is consistent with the hypothesis that DNA viruses might have been the central reservoir of inteins throughout the course of evolution.
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