The “Giant Virus Finder” discovers an abundance of giant viruses in the Antarctic dry valleys

@article{Kerepesi2017TheV,
  title={The “Giant Virus Finder” discovers an abundance of giant viruses in the Antarctic dry valleys},
  author={Csaba Kerepesi and Vince Korn{\'e}l Grolmusz},
  journal={Archives of Virology},
  year={2017},
  volume={162},
  pages={1671-1676}
}
Mimivirus was identified in 2003 from a biofilm of an industrial water-cooling tower in England. Later, numerous new giant viruses were found in oceans and freshwater habitats, some of them having 2,500 genes. We have demonstrated their likely presence in four soil samples taken from the Kutch Desert (Gujarat, India). Here we describe a bioinformatics work-flow, called the “Giant Virus Finder” that is capable of discovering the likely presence of the genomes of giant viruses in metagenomic… 

Giant viruses of the Kutch Desert

The Kutch Desert (Great Rann of Kutch, Gujarat, India) is a unique ecosystem: in the larger part of the year it is a hot, salty desert that is flooded regularly in the Indian monsoon season. In the

Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica

The isolation of 68 giant viruses of amoeba obtained from environmental samples from Brazil and Antarctica are described, thus being the first mimiviruses isolated in this extreme environment to date.

Advantages and Limits of Metagenomic Assembly and Binning of a Giant Virus

The results indicate that genome-resolved metagenomics is a valid approach for the recovery of near-complete giant virus genomes given that sufficient clonal particles are present, however, the data also underline that the vast majority of giant viruses remain currently undetected, even in an era of terabase-scale meetagenomics.

Giant virus biology and diversity in the era of genome-resolved metagenomics

How metagenomics has revolutionized the authors' perspective of giant viruses by revealing their distribution across their planet’s biomes, where they impact the biology and ecology of a wide range of eukaryotic hosts and ultimately affect global nutrient cycles is discussed.

Giant mimiviruses escape many canonical criteria of the virus definition.

Virophages of Giant Viruses: An Update at Eleven

Their parasitic lifestyle as bona fide viruses of the giant virus factories, genetic parasites of their genomes, and then their role as a key component or target for some host defense mechanisms during the tripartite virophage–giant virus–host cell interaction are discussed.

Boiling Acid Mimics Intracellular Giant Virus Genome Release

This work generated four metastable infection intermediates in Samba virus and generated proteins that are released from Samba and newly discovered Tupanvirus through differential mass spectrometry, and revealed the molecular forces that trigger infection are conserved amongst disparate giant viruses.

A Large Open Pangenome and a Small Core Genome for Giant Pandoraviruses

Phylogenomic analyses showed that P. massiliensis and P. braziliensis were more closely related to each other than to the other pandoraviruses, and of their relationships with viruses and cellular microorganisms.

References

SHOWING 1-10 OF 25 REFERENCES

Giant viruses of the Kutch Desert

The Kutch Desert (Great Rann of Kutch, Gujarat, India) is a unique ecosystem: in the larger part of the year it is a hot, salty desert that is flooded regularly in the Indian monsoon season. In the

Giant Marseillevirus highlights the role of amoebae as a melting pot in emergence of chimeric microorganisms

It is proposed that amoebae are “melting pots” of microbial evolution where diverse forms emerge, including giant viruses with complex gene repertoires of various origins.

Giant virus with a remarkable complement of genes infects marine zooplankton

Phylogenetic analysis indicates that CroV is a nucleocytoplasmic large DNA virus, with Acanthamoeba polyphaga mimivirus as its closest relative, although less than one-third of the genes of CroV have homologs in Mimivirus.

Virophage control of antarctic algal host–virus dynamics

It is determined that the virophage stimulates secondary production through the microbial loop by reducing overall mortality of the host and increasing the frequency of blooms during polar summer light periods.

The Giant Cafeteria roenbergensis Virus That Infects a Widespread Marine Phagocytic Protist Is a New Member of the Fourth Domain of Life

Analysis of the gene repertoire of CroV consolidated the fourth domain of Life hypothesis and contributed to outline a functional pan-genome for giant viruses infecting phagocytic protistan grazers.

Reclassification of Giant Viruses Composing a Fourth Domain of Life in the New Order Megavirales

It is proposed here to define a new viral order named Megavirales, which is shown to be monophyletic based on analyses of their sequences and gene repertoires and to contradict or do not match the criteria for the canonical definition of viruses.

Genomics of Megavirus and the elusive fourth domain of Life

Megavirus retained all of the genomic features unique to Mimivirus, in particular its genes encoding key-elements of the translation apparatus, a trademark of cellular organisms, and strongly suggests that large DNA viruses derived from an ancestral cellular genome by reductive evolution.

Informational Gene Phylogenies Do Not Support a Fourth Domain of Life for Nucleocytoplasmic Large DNA Viruses

It is shown that these informational gene alignments contain both significant compositional heterogeneity and homoplasy, which were not adequately modelled in the original analysis, and suggests that a fourth domain is not required to explain the available sequence data.

Pandoraviruses: Amoeba Viruses with Genomes Up to 2.5 Mb Reaching That of Parasitic Eukaryotes

The isolation of two giant viruses, one off the coast of central Chile, the other from a freshwater pond near Melbourne, without morphological or genomic resemblance to any previously defined virus families are reported.