The α-subunit regulates stability of the metal ion at the ligand-associated metal ion-binding site in β3 integrins.

@article{Rui2014TheR,
  title={The α-subunit regulates stability of the metal ion at the ligand-associated metal ion-binding site in β3 integrins.},
  author={Xianliang Rui and Mehrdad Mehrbod and Johannes F. Van Agthoven and Saurabh Anand and Jian Xiong and Mohammad R. K. Mofrad and M. Amin Arnaout},
  journal={The Journal of biological chemistry},
  year={2014},
  volume={289 33},
  pages={23256-63}
}
The aspartate in the prototypical integrin-binding motif Arg-Gly-Asp binds the integrin βA domain of the β-subunit through a divalent cation at the metal ion-dependent adhesion site (MIDAS). An auxiliary metal ion at a ligand-associated metal ion-binding site (LIMBS) stabilizes the metal ion at MIDAS. LIMBS contacts distinct residues in the α-subunits of the two β3 integrins αIIbβ3 and αVβ3, but a potential role of this interaction on stability of the metal ion at LIMBS in β3 integrins has not… CONTINUE READING