The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences.

@article{Donnelly2001TheA,
  title={The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences.},
  author={Marydele Donnelly and Lorraine E. Hughes and Graham N Luke and Hector Mendoza and E ten Dam and David Widjaja Gani and Martin D Ryan},
  journal={The Journal of general virology},
  year={2001},
  volume={82 Pt 5},
  pages={
          1027-41
        }
}
The 2A/2B cleavage of aphtho- and cardiovirus 2A polyproteins is mediated by their 2A proteins 'cleaving' at their own C termini. We have analysed this activity using artificial reporter polyprotein systems comprising green fluorescent protein (GFP) linked via foot-and-mouth disease virus (FMDV) 2A to beta-glucuronidase (GUS) -- forming a single, long, open reading frame. Analysis of the distribution of radiolabel showed a high proportion of the in vitro translation products (approximately 90… CONTINUE READING

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The cleavage activity of aphtho- and cardiovirus 2A proteins

  • M.L.L. Donnelly, D. Gani, M. Flint, S. Monoghan, M. D. Ryan
  • Journal of General Virology
  • 1997
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