Thawed human hepatocytes in primary culture.

@article{Dou1992ThawedHH,
  title={Thawed human hepatocytes in primary culture.},
  author={M Dou and Georges de Sousa and Bruno Lacarelle and Michel Placidi and P Lech{\^e}ne de la Porte and Maria Domingo and H. Lafont and Roger Rahmani},
  journal={Cryobiology},
  year={1992},
  volume={29 4},
  pages={454-69}
}
In drug metabolism studies, isolated and cultured human hepatocytes provide a useful model for overcoming the difficulty of extrapolating from animal data. In vitro studies with human hepatocytes are scarce because of the lack of livers and suitable methods of storage. After developing a new method for cryopreservation of human hepatocytes, we evaluated the effects of deep freezing storage on their viability, morphology, and functional and toxicological capabilities in classical culture… CONTINUE READING
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