Textured soy protein scaffolds enable the generation of three-dimensional bovine skeletal muscle tissue for cell-based meat

  title={Textured soy protein scaffolds enable the generation of three-dimensional bovine skeletal muscle tissue for cell-based meat},
  author={Tom Ben-Arye and Yulia Shandalov and Shahar Ben-Shaul and Shira Landau and Yedidya Zagury and Iris Ianovici and Neta Lavon and Shulamit Levenberg},
Cell-based meat (CBM) production is a promising technology that could generate meat without the need of animal agriculture. The generation of tissue requires a three-dimensional (3D) scaffold to provide support to the cells and mimic the extracellular matrix (ECM). For CBM, the scaffold needs to be edible and have suitable nutritional value and texture. Here, we demonstrate the use of textured soy protein—an edible porous protein-based biomaterial—as a novel CBM scaffold that can support cell… 
Extracellular Matrix and the Production of Cultured Meat
The influence of ECM on myogenesis and its role as a scaffold and vital component to improve the efficacy of the culture media used to produce cultured meat are explored.
Engineering Murine Adipocytes and Skeletal Muscle Cells in Meat-like Constructs Using Self-Assembled Layer-by-Layer Biofabrication: A Platform for Development of Cultivated Meat
A scaffold-free biofabrication method is demonstrated that forms cell sheets composed of murine adipocytes and skeletal muscle cells and assembles these sheets in parallel to create a 3D meat-like construct without the use of any exogenous materials.
Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
It is shown that fibro-adipogenic progenitor cells (FAPs) are present in bovine muscle, and are transcriptionally and immunophenotypically distinct from satellite cells, and they represent a promising candidate cell type for the production of cultured fat.
Scaffolds for Cultured Meat on the Basis of Polysaccharide Hydrogels Enriched with Plant-Based Proteins
The world population is growing and alternative ways of satisfying the increasing demand for meat are being explored, such as using animal cells for the fabrication of cultured meat. Edible
Engineered whole cut meat-like tissue by the assembly of cell fibers using tendon-gel integrated bioprinting
In this study, in vitro construction of engineered steak-like tissue assembled of three types of bovine cell fibers (muscle, fat, and vessel) is demonstrated.


Tissue Engineering for Clean Meat Production
This review focuses on tissue engineering of skeletal muscle and the adjustments needed for clean meat development, and elaborate on cell types from farm animals that have the potential to recapitulate the muscle ECM, blood vessels, muscle fibers and fat deposits.
Meat Science and Muscle Biology Symposium: manipulating mesenchymal progenitor cell differentiation to optimize performance and carcass value of beef cattle.
Changing mesenchymal progenitor cell differentiation through nutritional management of cows, or fetal programming, is a promising method to improve cattle performance and carcass value.
Bovine myoblast cell production in a microcarriers-based system
This results provide valuable data to further develop and scale-up the production of bovine myoblasts as a prerequisite for efficient and cost-effective development of cultured meat.
Adipogenesis from Bovine Precursors.
This work presents a protocol for the isolation of adipose tissue-derived preadipocytes and subsequent adipogenic differentiation through free fatty acid stimulation, and delineation is visually confirmed through lipophilic staining.
3D skeletal muscle fascicle engineering is improved with TGF-β1 treatment of myogenic cells and their co-culture with myofibroblasts
Results show skeletal muscle regeneration can be promoted by treating myogenic cells with TGF-β1, and myofibroblasts are superior enhancers of myogenesis than fibroblast, and both TGFs can serve as myogenesis accelerators across multiple tissue engineering platforms.
Substrate elasticity affects bovine satellite cell activation kinetics in vitro.
The results confirm that biophysical and biochemical signals regulate BSC activation and that with increasing surface elasticity, an increase in the numbers of muscle progenitors was observed.
Fibrin gel -- advantages of a new scaffold in cardiovascular tissue engineering.
  • S. Jockenhoevel, G. Zund, M. Turina
  • Medicine, Biology
    European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery
  • 2001
Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle.
Tissue-engineered skeletal muscle consisting of human aligned myofibers with interspersed endothelial networks is reported, which allows for a physiologically more relevant muscle model and paves the way toward engineering of larger in vitro muscle constructs.
Three-dimensional scaffolds for tissue engineering applications: role of porosity and pore size.
The ability of pore size and porosity of scaffolds to direct cellular responses and alter the mechanical properties of scaffold will be reviewed, followed by a look at nature's own scaffold, the extracellular matrix.