Tetracycline-Inducible System for Regulation of Skeletal Muscle-Specific Gene Expression in Transgenic Mice

@article{Grill2003TetracyclineInducibleSF,
  title={Tetracycline-Inducible System for Regulation of Skeletal Muscle-Specific Gene Expression in Transgenic Mice},
  author={Mischala A. Grill and Mark A. Bales and Amber N. Fought and Kristopher C. Rosburg and Stephanie J. Munger and Parker B. Antin},
  journal={Transgenic Research},
  year={2003},
  volume={12},
  pages={33-43}
}
Tightly regulated control of over-expression is often necessary to study one aspect or time point of gene function and, in transgenesis, may help to avoid lethal effects and complications caused by ubiquitous over-expression. We have utilized the benefits of an optimized tet-on system and a modified muscle creatine kinase (MCK) promoter to generate a skeletal muscle-specific, doxycycline (Dox) controlled over-expression system in transgenic mice. A DNA construct was generated in which the codon… CONTINUE READING