Tetracycline: Effect on Osteogenesis in Vitro.

  title={Tetracycline: Effect on Osteogenesis in Vitro.},
  author={Lauri Sax{\'e}n},
  volume={149 3686},
Tetracycline hydrochloride added to cultures of rudiments of embryonic bone in a concentration of 1 microgram per milliliter prevents mineralization of the bones and is found throughout the calcified zones. The inhibition is reversible; upon transfer of a treated culture to a normal medium, mineralization proceeds normally but maldeveloped bones result.