Tetrachloroethylene, trichloroethylene, and chlorinated phenols induce toluene-o-xylene monooxygenase activity in Pseudomonas stutzeri OX1

  title={Tetrachloroethylene, trichloroethylene, and chlorinated phenols induce toluene-o-xylene monooxygenase activity in Pseudomonas stutzeri OX1},
  author={Doohyun Ryoo and Hojae Shim and Fabio L. G. Arenghi and Paola Barbieri and Thomas M Wood},
  journal={Applied Microbiology and Biotechnology},
  • D. Ryoo, H. Shim, T. Wood
  • Published 1 August 2001
  • Chemistry
  • Applied Microbiology and Biotechnology
Abstract.Pseudomonas stutzeri OX1 naphthalene-oxidation activity is induced 3.0-fold by tetrachloroethylene (PCE) and 3.1-fold by trichloroethylene (TCE) at 100 µM. With the mutant P. stutzeri M1, which does not express toluene-o-xylene monooxygenase (ToMO, product of the tou operon), no naphthalene-oxidation activity induction by PCE and TCE was found; hence, PCE and TCE induce ToMO of P. stutzeri OX1. The chlorinated phenols 2-, 3-, and 4-chlorophenol induced ToMO expression 0.58-, 0.23- and… 
Protein engineering of toluene-o-xylene monooxygenase from Pseudomonas stutzeri OX1 for enhanced chlorinated ethene degradation and o-xylene oxidation
DNA shuffling of the alpha hydroxylase fragment of ToMO (TouA) and saturation mutagenesis of the TouA active site residues I100, Q141, T201, F205, and E214 were used to enhance the degradation of chlorinated aliphatics.
Aerobic degradation of mixtures of tetrachloroethylene, trichloroethylene, dichloroethylenes, and vinyl chloride by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1
This indicates that for the first time an aerobic enzyme can degrade mixtures of all chlorinated ethenes, including the once – so it was believed – completely recalcitrant PCE.
Chemotaxis of Pseudomonas stutzeri OX1 and Burkholderia cepacia G4 toward chlorinated ethenes
The chemotactic responses of Pseudomonas putida F1, Burkholderia cepacia G4, and Pseudomonas stutzeri OX1 were investigated toward toluene, trichloroethylene (TCE), tetrachloroethylene (PCE),
Removal of mixture of cis‐1,2‐dichloroethylene/trichloroethylene/benzene, toluene, ethylbenzene, and xylenes from contaminated soil by Pseudomonas plecoglossicida
BACKGROUND This study focused on the simultaneous removal of BTEX (benzene, toluene, ethylbenzene, and xylenes) and CAHs (chlorinated aliphatic hydrocarbons, represented by cis-DCE
Effects of Dichloroethene Isomers on the Induction and Activity of Butane Monooxygenase in the Alkane-Oxidizing Bacterium “Pseudomonas butanovora”
1,2-trans DCE was unique among the BMO substrates tested in its ability to induce lacZ expression, and supported greater rates of oxidation of the three DCEs than the other organic acids tested.
Effect of toluene concentration and hydrogen peroxide on Pseudomonasplecoglossicida cometabolizing mixture of cis-DCE and TCE in soil slurry
An indigenous Pseudomonas sp., isolated from the regional contaminated soil and identified as P. plecoglossicida, was evaluated for its aerobic cometabolic removal of cis-1,2-dichloroethylene
Induction of Methyl Tertiary Butyl Ether (MTBE)-Oxidizing Activity in Mycobacterium vaccae JOB5 by MTBE
Experiments with acid-grown cells suggested induction of MTBE-oxidizing activity by MTBE is subject to catabolite repression, and the results of this study are discussed in terms of their potential implications towards the understanding of the role of cometabolism in MTBE and TBA biodegradation in gasoline-contaminated environments.
Gasoline Oxygenate Biodegradation Processes in Mycobacterium vaccae JOB5
This study aimed to investigate the monooxygenase in Mycobacterium vaccae JOB5 previously found to cometabolize MTBE, focusing on the regulation and substrate range of this enzyme, and led to the expansion of known n-alkanes which support growth in this organism to include the gaseous alkanes, n-butane and propane.


Oxidation of Trichloroethylene, 1,1-Dichloroethylene, and Chloroform by Toluene/o-Xylene Monooxygenase from Pseudomonas stutzeri OX1
Escherichia coli JM109 that expressed ToMO from genes on plasmid pBZ1260 under control of the lac promoter degraded TCE, 1,1-DCE, and chloroform at initial rates, extending the substrate range of ToMO to include aliphatic chlorinated compounds.
Aerobic degradation of mixtures of chlorinated aliphatics by cloned toluene-o-xylene monooxygenase and toluene o-monooxygenase in resting cells.
In mixtures of these chlorinated compounds, degradation was found to occur simultaneously rather than sequentially, and the mineralization of many of these compounds could be confirmed through detection of chloride ions.
Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1
It is reported that the wastewater bacterium Pseudomonas stutzeri OX1 degrades aerobically 0.56 μmol of 2.0 μmol PCE in 21 h (Vmax ≈ 2.5 nmol min−1 mg−1 protein and KM ≈ 34 μM).
Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase
Degradation of trichloroethylene (TCE) by the methanotrophic bacterium Methylosinus trichosporium OB3b was studied by using cells grown in continuous culture. TCE degradation was a strictly
Isolation of a Pseudomonas stutzeri strain that degrades o-xylene
The suggested hypothesis is that the first metabolic steps of growth on o-xylene involve the direct oxygenation of the aromatic nucleus, followed by meta pathway reactions.
Activation and Inactivation of Pseudomonas stutzeriMethylbenzene Catabolism Pathways Mediated by a Transposable Element
A 3-kb insertion sequence (IS) termed ISPs1, which inactivates them-xylene and p-Xylene catabolic pathway in P. stutzeri OX1 and the o-xene catabolic genes inP.
Trichloroethylene oxidation by purified toluene 2-monooxygenase: products, kinetics, and turnover-dependent inactivation
The addition of cysteine to reaction mixtures partially protected the enzyme system against inactivation, most notably protecting the NADH-oxidoreductase component.
Comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria
The results of experiments performed with strains F1, G4, PKO1, and KR1 suggested that T CE or an oxidation product induces toluene degradation and that TCE induces its own degradation in the monooxygenase strains, and this was confirmed by performing a promoter probe analysis.
Analysis of the Gene Cluster Encoding Toluene/o-Xylene Monooxygenase from Pseudomonas stutzeri OX1
The sequence analysis revealed the presence of six open reading frames (ORFs) homologous to other genes clustered in operons coding for multicomponent monooxygenases found in benzene- and toluene-degradative pathways cloned from Pseudomonas strains, and showed that all six ORFs are essential for the full activity of the toLUene/o-xylene monooxyGENase in Escherichia coli.
Transformation Kinetics of Chlorinated Ethenes by Methylosinus trichosporium OB3b and Detection of Unstable Epoxides by On-Line Gas Chromatography
A rapid and accurate method for the determination of transformation kinetics of volatile organic substrates was developed and Methane and acetylene inhibited degradation of the epoxide of cis-1,2-dichloroethene, indicating that sMMO was involved.