Tests of a model of specific contacts in T7 RNA polymerase-promoter interactions.

@article{Schick1995TestsOA,
  title={Tests of a model of specific contacts in T7 RNA polymerase-promoter interactions.},
  author={Charles Schick and Craig T. Martin},
  journal={Biochemistry},
  year={1995},
  volume={34 2},
  pages={666-72}
}
The T7, T3, and SP6 RNA polymerases represent a highly homologous family of enzymes that recognize similarly homologous promoter DNA sequences. Despite these similarities, the enzymes are highly specific for their respective promoters. Studies of mutant RNA polymerases have linked a specific amino acid residue in the protein to recognition of bases at positions -11 and -10 in the promoter [Raskin, C. A., et al. (1992) J. Mol. Biol. 228, 506-515]. In kinetic analyses of transcription from… CONTINUE READING