Testosterone regulates apoptosis in adult human seminiferous tubules in vitro.

@article{Erkkil1997TestosteroneRA,
  title={Testosterone regulates apoptosis in adult human seminiferous tubules in vitro.},
  author={Krista Erkkil{\"a} and Kristin Henriksen and Virve Hirvonen and Sakari Rannikko and Jarmo Salo and Martti Parvinen and Leo Dunkel},
  journal={The Journal of clinical endocrinology and metabolism},
  year={1997},
  volume={82 7},
  pages={2314-21}
}
In the present study an in vitro model was developed and characterized for evaluation of the role of apoptosis in adult human testes. The samples came from adult men undergoing orchidectomy for prostate or testicular cancer. Segments of seminiferous tubules were isolated and incubated under serum-free conditions in the absence or presence of testosterone. Apoptosis was assessed by low mol wt DNA fragmentation (185-bp multiples) by use of 3'-end-labeled DNA, in situ end labeling, and… CONTINUE READING