Testosterone metabolism in primary cultures of epithelial cells and stroma from benign prostatic hyperplasia

@article{Tsugaya1996TestosteroneMI,
  title={Testosterone metabolism in primary cultures of epithelial cells and stroma from benign prostatic hyperplasia},
  author={Masayuki Tsugaya and Fouad K. Habib and Geoffrey D. Chisholm and Margaret R. Ross and Keiichi Tozawa and Yoshihiko Hayashi and Kenjiro Kohri and Seiichi Tanaka},
  journal={Urological Research},
  year={1996},
  volume={24},
  pages={265-271}
}
We studied the metabolism of testosterone in primary cultures of prostate epithelial cells and fibroblasts obtained from patients with benign prostatic hyperplasia (BPH). The conversion of 3H-testosterone in both cell cultures was predominantly to the oxidative pathway, with the formation of 3H-androstenedione increasing with cell number and time of incubation. Although we also detected some 5α-reductase activity in these cells, the activity in the stroma component (0.00688 pmol/mg protein/min… CONTINUE READING