Testing the conservation of the translational machinery over evolution in diverse environments: assaying Thermus thermophilus ribosomes and initiation factors in a coupled transcription-translation system from Escherichia coli.

@article{Thompson2004TestingTC,
  title={Testing the conservation of the translational machinery over evolution in diverse environments: assaying Thermus thermophilus ribosomes and initiation factors in a coupled transcription-translation system from Escherichia coli.},
  author={Jill Thompson and Albert E. Dahlberg},
  journal={Nucleic acids research},
  year={2004},
  volume={32 19},
  pages={5954-61}
}
Ribosomes from the extreme thermophile Thermus thermophilus are capable of translation in a coupled transcription-translation system derived from Escherichia coli. At 45 degrees C, T.thermophilus ribosomes translate at approximately 25-30% of the maximal rate of E.coli ribosomes, and synthesize full-length protein. T.thermophilus and E.coli subunits can be combined to effect translation, with the spectrum of proteins produced depending upon the source of the 30S subunit. In this system, T… CONTINUE READING

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Electron microscopy of functional ribosome complexes

J. Frank
Biopolymers • 2003

Crystal structure of an initiation factor bound to the 30S ribosomal subunit

A. P. Carter, Clemons, +5 authors V. Ramakrishnan
Science • 2001

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