Testing for intron function in the essential Saccharomyces cerevisiae tRNA(SerUCG) gene.

Abstract

The gene sup61+, which codes for the essential Saccharomyces cerevisiae tRNA(SerUCG), is the only single-copy tRNA gene in this organism know to contain an intron. To assess the role of this intron in tRNA gene expression, an intron-deleted sup61+ gene was constructed in vitro and introduced into the yeast genome. Isogenic intron- and intron+ strains were found to be indistinguishable by criteria that include growth rates, ability to undergo meiosis, levels of mature tRNA(SerUCG) transcribed in vivo, and the suppressor efficiency of amber- and ochre-specific alleles of this gene.

Cite this paper

@article{Ho1988TestingFI, title={Testing for intron function in the essential Saccharomyces cerevisiae tRNA(SerUCG) gene.}, author={Calvin K Ho and Megan Mayerle}, journal={Journal of molecular biology}, year={1988}, volume={202 3}, pages={667-72} }