Testicular hyaluronidase induces tubular structures of endothelial cells grown in three‐dimensional colagen gel through a CD44‐mediated mechanism

@article{Rahmanian2002TesticularHI,
  title={Testicular hyaluronidase induces tubular structures of endothelial cells grown in three‐dimensional colagen gel through a CD44‐mediated mechanism},
  author={Mehdi Rahmanian and Paraskevi Heldin},
  journal={International Journal of Cancer},
  year={2002},
  volume={97}
}
Cultured brain capillary endothelial cells grown in a 3‐dimensional collagen gel can form tubular structures after stimulation by angiogenic factors. We found that treatment of such cultures with testicular hyaluronidase led to formation of tubular structures and cell survival. Anion‐exchange chromatography of the enzyme preparation on a MonoQ column revealed the presence of the angiogenic factor basic fibroblast growth factor (bFGF) in the flow‐through fraction, as determined by immunoblotting… Expand
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