Test of synergistic interactions among deleterious mutations in bacteria

@article{Elena1997TestOS,
  title={Test of synergistic interactions among deleterious mutations in bacteria},
  author={Santiago F. Elena and Richard E. Lenski},
  journal={Nature},
  year={1997},
  volume={390},
  pages={395-398}
}
Identifying the forces responsible for the origin and maintenance of sexuality remains one of the greatest unsolved problems in biology. The mutational deterministic hypothesis postulates that sex is an adaptation that allows deleterious mutations to be purged from the genome; it requires synergistic interactions, which means that two mutations would be more harmful together than expected from their separate effects,. We generated 225 genotypes of Escherichia coli carrying one, two or three… Expand
Epistatic interactions of spontaneous mutations in haploid strains of the yeast Saccharomyces cerevisiae
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Epistasis between Beneficial Mutations and the Phenotype-to-Fitness Map for a ssDNA Virus
TLDR
A simple model is developed in which the phenotypic effects of mutations are completely additive and epistatic interactions arise as a result of the form of the phenotype-to-fitness mapping, which provided a good explanation for the data and the observed patterns of epistatic interaction. Expand
EPISTASIS BETWEEN NEW MUTATIONS AND GENETIC BACKGROUND AND A TEST OF GENETIC CANALIZATION
  • S. Elena, R. Lenski
  • Biology, Medicine
  • Evolution; international journal of organic evolution
  • 2001
TLDR
Interactions between pairs of random insertion mutations in the bacterium Escherichia coli are examined by transducing each of twelve insertion mutations into two genetic backgrounds, one ancestral and the other having evolved in, and adapted to, a defined laboratory environment for 10,000 generations. Expand
TESTING FOR EPISTASIS BETWEEN DELETERIOUS MUTATIONS IN A PARASITOID WASP
TLDR
This work examines two components of fitness, both of which are thought to be important in natural populations of parasitic wasps: longevity and egg production, and shows synergistic epistasis for longevity, but not for egg production. Expand
Mutation-selection balance, dominance and the maintenance of sex.
TLDR
A significant advantage of sex due to nearly recessive mutations provided the mutation rate per genome is of order one, by reconsidering the theory of mutation-selection balance at a single diploid gene locus. Expand
Genomic buffering mitigates the effects of deleterious mutations in bacteria
TLDR
This work measured the decrease in fitness caused by increasing mutation number in the bacterium Salmonella typhimurium using a regulated, error-prone DNA polymerase and found that as mutations accumulated, fitness costs increased at a diminishing rate. Expand
Mutation and sex in a competitive world
TLDR
A mathematical model of competition for food in which deleterious mutations affect competitive ability is analysed, showing that, if individuals usually compete in small groups, then competition can easily lead to a type of genetic interaction known as synergistic epistasis. Expand
The contribution of epistasis to the architecture of fitness in an RNA virus.
  • R. Sanjuán, A. Moya, S. Elena
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2004
TLDR
The results show that the architecture of the fitness depends on complex interactions among genome components, including antagonistic and synergistic epistasis. Expand
Testing for epistasis between deleterious mutations.
TLDR
It is shown that only one of these methods is likely to give useful results: crossing individuals that have very different numbers of deleterious mutations, and comparing the mean log fitness of the parents with that of their offspring. Expand
Dependence of epistasis on environment and mutation severity as revealed by in silico mutagenesis of phage t7.
TLDR
This work employed a computer model for the intracellular growth of bacteriophage T7 to demonstrate how computer simulations of developmental processes can be used to quantitatively study genetic interactions at the population level and suggests that synergistic epistasis may be difficult to experimentally distinguish from nonepistasis. Expand
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