TerraGenome: a consortium for the sequencing of a soil metagenome

  title={TerraGenome: a consortium for the sequencing of a soil metagenome},
  author={Timothy M. Vogel and Pascal Simonet and Janet K. Jansson and Penny R. Hirsch and James M. Tiedje and Jan Dirk van Elsas and Mark J. Bailey and Renaud Nalin and Laurent Philippot},
  journal={Nature Reviews Microbiology},
Vogel and colleagues invite the microbiology community to participate in an ambitious and extraordinary sequencing project to uncover the soil metagenome. 

Estimation of metagenome size and structure in an experimental soil microbiota from low coverage next‐generation sequence data

A novel approach to estimate the size of all combined genomes for low coverage next‐generation sequencing (NGS) data through empirically determined copy numbers of random DNA fragments is presented.

Recent Advances and Perspectives in Metagenomic Studies of Soil Microbial Communities

The advent of high-throughput sequencing techniques had opened the principally new opportunities in studies of the genetic structure of microbial communities, but at the same time highlighted significant difficulties, arising particularly during the investigation of the soil metagenome.

Identification and characterisation of resistance to the take-all fungus in wheat

This project was funded by a CASE PhD studentship from the Biotechnology and Biological Sciences Research Council (BBSRC), with industrial support from HGCA.

Advantages of the metagenomic approach for soil exploration: reply from Vogel et al.

Systems insights will be obtained and the prospect of completely sequencing a soil metagenome realistic is made realistic as the majority of the microorganisms in soil cannot yet be grown in the laboratory.

The -Omics Race

A number of advancements concerning the genome organization and functioning of the bacterial chromosome are reviewed, including the genome GC content, gene ordering and classification, and the

Novel Florfenicol and Chloramphenicol Resistance Gene Discovered in Alaskan Soil by Using Functional Metagenomics

A gene that mediated reduced susceptibility to florfenicol was identified and designated pexA, and the predicted PexA protein showed a structure similar to that of efflux pumps of the major facilitator superfamily.

Soil genomics

The recent editorial on the TerraGenome consortium by Vogel et al. elegantly highlighted the needs, requirements and challenges for sequencing the genomes of soil microorganisms in line with human genome and marine metagenomic projects.

Predominant but Previously-overlooked Prokaryotic Drivers of Reductive Nitrogen Transformation in Paddy Soils, Revealed by Metatranscriptomics

A shotgun RNA sequencing analysis (metatranscriptomics) of paddy soil samples revealed that most RNT gene transcripts in paddy soils were derived from Deltaproteobacteria, particularly the genera Anaeromyxobacter and Geobacter.

From bacterial to microbial ecosystems (metagenomics).

The different components of a metagenomic study including sample collection, DNA extraction, sequencing, and informatics are discussed, which highlight their issues and challenges, and review the solutions that are currently in use.



The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific

A metagenomic study of the marine planktonic microbiota in which surface (mostly marine) water samples were analyzed as part of the Sorcerer II Global Ocean Sampling expedition, which yielded an extensive dataset consisting of 7.7 million sequencing reads.

Finishing the euchromatic sequence of the human genome

The near-complete sequence reported here should serve as a firm foundation for biomedical research in the decades ahead and greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death.