Terminal restriction pattern analysis of 16S rRNA genes for the characterization of bacterial communities of activated sludge.

@article{Hiraishi2000TerminalRP,
  title={Terminal restriction pattern analysis of 16S rRNA genes for the characterization of bacterial communities of activated sludge.},
  author={Akira Hiraishi and Mitsuru Iwasaki and Hidetaka Shinjo},
  journal={Journal of bioscience and bioengineering},
  year={2000},
  volume={90 2},
  pages={148-56}
}
A culture-independent molecular technique using terminal restriction fragment length polymorphism (T-RFLP) of 16S rRNA genes was applied to the characterization of bacterial communities of activated sludge taken from different municipal sewage treatment plants. 16S rDNA fragments from the bulk DNA of sludge were amplified by PCR with a Cy5-labeled forward primer corresponding to nucleotide positions 8 to 27 and a reverse primer complementary to positions 907 to 926 in the Escherichia coli… CONTINUE READING

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