Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point.

@article{Bukovsk2010TerminalRF,
  title={Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point.},
  author={Petra Bukovsk{\'a} and Mark{\'e}ta Jel{\'i}nkov{\'a} and Hana Hr{\vs}elov{\'a} and Zuzana S{\'y}korov{\'a} and Milan Gryndler},
  journal={Journal of microbiological methods},
  year={2010},
  volume={82 3},
  pages={223-8}
}
Several methods of molecular analysis of microbial diversity, including terminal restriction fragment length polymorphism (T-RFLP) analysis are based on measurement of the DNA fragment length. Significant variation between sequence-determined and measured length of restriction fragments (drift) has been observed, which can affect the efficiency of the identification of microorganisms in the analyzed communities. In the past, this variation has been attributed to varying fragment length and… CONTINUE READING

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